Literature summary for 6.2.1.72 extracted from

Reichert, J.; Sakaitani, M.; Walsh, C.T.
Characterization of EntF as a serine-activating enzyme (1992), Protein Sci., 1, 549-556 .

Search for more literature for 6.2.1.72

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(L-seryl)adenylate + holo-[L-seryl-carrier protein]	Escherichia coli	-	AMP + L-seryl-[L-seryl-carrier protein]	-	?
ATP + L-serine	Escherichia coli	-	diphosphate + (L-seryl)adenylate	-	?
ATP + L-serine + holo-[L-seryl-carrier protein]	Escherichia coli	-	AMP + diphosphate + L-seryl-[L-seryl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P11454	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, by initial ammonium sulfate precipitation followed by a single Fast-Flow Q ion exchange column	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(L-seryl)adenylate + holo-[L-seryl-carrier protein]	-	Escherichia coli	AMP + L-seryl-[L-seryl-carrier protein]	-	?
ATP + L-serine	-	Escherichia coli	diphosphate + (L-seryl)adenylate	-	?
ATP + L-serine + holo-[L-seryl-carrier protein]	-	Escherichia coli	AMP + diphosphate + L-seryl-[L-seryl-carrier protein]	-	?
additional information	enzyme exhibits L-serine-dependent ATP[32P] diphosphate exchange activity with a Km for serine of 260 microM and a turnover number of 12.6 per s	Escherichia coli	?	-	-

Synonyms

Synonyms	Comment	Organism
enterobactin synthase component F	-	Escherichia coli
entF	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	EntF is the enzyme responsible for serine activation during the biosynthesis of enterobactin in Escherichia coli	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)