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Literature summary for 6.2.1.72 extracted from

  • Ehmann, D.; Shaw-Reid, C.; Losey, H.; Walsh, C.
    The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase Sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates (2000), Proc. Natl. Acad. Sci. USA, 97, 2509-2514 .
    View publication on PubMed
Search for more literature for 6.2.1.72

Organism

Organism UniProt Comment Textmining
Escherichia coli P11454
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-seryl)adenylate + holo-[L-seryl-carrier protein]
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 Escherichia coli AMP + L-seryl-[L-seryl-carrier protein]
-
 ?
ATP + L-serine
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 Escherichia coli diphosphate + (L-seryl)adenylate
-
 ?
ATP + L-serine + holo-[L-seryl-carrier protein domain of EntF]
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 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of EntF]
-
 ?
ATP + L-serine + holo-[L-seryl-carrier protein domain of SrfB1]
-
 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of SrfB1]
-
 ?
ATP + L-serine + holo-[L-seryl-carrier protein domain of Ybt]
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 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of Ybt]
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 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00167
-
 L-serine acceptor holo-[L-seryl-carrier protein domain of Ybt], pH 7.5, 37°C Escherichia coli
0.0083
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 L-serine acceptor holo-[L-seryl-carrier protein domain of SrfB1], pH 7.5, 37°C Escherichia coli

General Information

General Information Comment Organism
physiological function 142-kDa four-domain protein EntF is bisected into two double-domain fragments: a 108-kDa condensation and adenylation construct, EntF C-A, and a 37-kDa peptidyl carrier protein (PCP) and thioesterase protein, EntF PCP-TE. The adenylation domain activity of EntF C-A forms seryl-AMP but has lost the ability to transfer the seryl moiety to the cognate EntF PCP-TE in trans. Seryl transfer to heterologous PCP protein fragments, the SrfB1 PCP from surfactin synthetase and Ybt PCP1 from yersiniabactin synthetase, is observed at rates of 0.5 per min and 0.01 per min, respectively. The holo SrfB1 PCP protein as well as Ybt PCP1 do not stimulate an increase in diphosphate release from EntF C-A or EntE Escherichia coli