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Literature summary for 6.2.1.70 extracted from
- SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase (2005), Proc. Natl. Acad. Sci. USA, 102, 10111-10116 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichioa coli | Pseudomonas syringae pv. syringae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
L-threonine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae |  |
| 7.7 | - |
L-serine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae pv. syringae | Q52400 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged protein | Pseudomonas syringae pv. syringae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (L-threonyl)adenylate + holo-[L-threonyl-carrier protein] | - |
Pseudomonas syringae pv. syringae | AMP + L-threonyl-[L-threonyl-carrier protein] | - |
? | |
| ATP + L-serine + holo-[L-threonyl-carrier protein] | - |
Pseudomonas syringae pv. syringae | AMP + diphosphate + L-seryl-[L-threonyl-carrier protein] | - |
? | |
| ATP + L-threonine | - |
Pseudomonas syringae pv. syringae | diphosphate + (L-threonyl)adenylate | - |
? | |
| ATP + L-threonine + holo-[L-threonyl-carrier protein] | - |
Pseudomonas syringae pv. syringae | AMP + diphosphate + L-threonyl-[L-threonyl-carrier protein] | - |
? | |
| additional information | less than 1% of the activity with L-Thr: 4-Cl-L-Thr, D-Thr, L-Ile, D-Ser (less than 1% of the activity with L-Thr each) | Pseudomonas syringae pv. syringae | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Pseudomonas syringae pv. syringae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| syrB1 | - |
Pseudomonas syringae pv. syringae |
| syringomycin biosynthesis enzyme 1 | - |
Pseudomonas syringae pv. syringae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
L-serine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae | |
| 0.485 | - |
L-threonine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
L-serine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae | |
| 0.16 | - |
L-threonine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas syringae pv. syringae | |
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