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Literature summary for 6.2.1.60 extracted from

  • Dunn, Z.; Wever, W.; Economou, N.; Bowers, A.; Li, B.
    Enzymatic basis of "hybridity" in thiomarinol biosynthesis (2015), Angew. Chem. Int. Ed. Engl., 54, 5137-5141 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the pair acetyltransferase HolE/TmlU can readily ligate pseudomonic acid C to a variety of primary amines including 3-aminocoumarins, but is less effective with a series of aryl amines. An adjacent substrate carbonyl appears useful or important for recognition of the amine donor Pseudoalteromonas sp. SANK 73390

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas sp. SANK 73390

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
pseudomonic acid C pH not specified in the publication, temperature not specified in the publication Pseudoalteromonas sp. SANK 73390

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + marinolic acid C + CoA Pseudoalteromonas sp. SANK 73390
-
AMP + diphosphate + marinoloyl C-CoA
-
?
ATP + octanoic acid + CoA Pseudoalteromonas sp. SANK 73390
-
AMP + diphosphate + octanoyl-CoA
-
?
ATP + pseudomonic acid A + CoA Pseudoalteromonas sp. SANK 73390
-
AMP + diphosphate + pseuodomonoyl A-CoA
-
?
ATP + pseudomonic acid C + CoA Pseudoalteromonas sp. SANK 73390
-
AMP + diphosphate + pseudomonoyl C-CoA
-
?
additional information Pseudoalteromonas sp. SANK 73390 at high enzyme concentration, TmlU is capable of activating acetic, octanoic, 2,4-dodecadienoic, and 2,4-decadienoic acids, albeit to a lesser extent ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. SANK 73390 F8J3D9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + marinolic acid C + CoA
-
Pseudoalteromonas sp. SANK 73390 AMP + diphosphate + marinoloyl C-CoA
-
?
ATP + octanoic acid + CoA
-
Pseudoalteromonas sp. SANK 73390 AMP + diphosphate + octanoyl-CoA
-
?
ATP + pseudomonic acid A + CoA
-
Pseudoalteromonas sp. SANK 73390 AMP + diphosphate + pseuodomonoyl A-CoA
-
?
ATP + pseudomonic acid C + CoA
-
Pseudoalteromonas sp. SANK 73390 AMP + diphosphate + pseudomonoyl C-CoA
-
?
additional information at high enzyme concentration, TmlU is capable of activating acetic, octanoic, 2,4-dodecadienoic, and 2,4-decadienoic acids, albeit to a lesser extent Pseudoalteromonas sp. SANK 73390 ?
-
?

Synonyms

Synonyms Comment Organism
tmlU
-
Pseudoalteromonas sp. SANK 73390

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
pseudomonic acid C pH not specified in the publication, temperature not specified in the publication Pseudoalteromonas sp. SANK 73390

General Information

General Information Comment Organism
physiological function TmlU acts as a CoA ligase that activates marinolic acid as its thioester before it is processed by the acetyltransferase HolE to catalyze the amidation. TmlU prefers complex acyl acids as substrates Pseudoalteromonas sp. SANK 73390