Literature summary for 6.2.1.54 extracted from

Du, L.; He, Y.; Luo, Y.
Crystal Structure and Enantiomer Selection by D-Alanyl Carrier Protein Ligase DltA from Bacillus cereus (2008), Biochemistry, 47, 11473-11480.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of a DltA is determined at 2.0 A resolution in complex with the D-alanine adenylate intermediate of the first reaction. Despite the low level of sequence similarity, the DltA structure resembles known structures of adenylation domains such as the acetyl-CoA synthetase. The enantiomer selection appears to be enhanced by the medium-sized side chain of Cys-269	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
C269A	C269A mutation relaxes D-Ala preference. Km (mM): (D-Ala) 3.1, (L-Ala) 6.6, kcat (1/sec): (D-Ala) 0.103, (L-Ala) 0.12	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	D-Ala	wild-tpye	Bacillus cereus
3.1	-	D-Ala	mutant C269A	Bacillus cereus
6.6	-	L-Ala	mutant C269A	Bacillus cereus
14.4	-	L-Ala	wild-tpye	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	Q81G39	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-Ala + diphosphate	-	Bacillus cereus	?	-	?
ATP + L-Ala + diphosphate	-	Bacillus cereus	?	-	?

Synonyms

Synonyms	Comment	Organism
BcDltA	-	Bacillus cereus
D-alanyl carrier protein ligase	-	Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.025	-	D-Ala	wild-tpye	Bacillus cereus
0.103	-	D-Ala	mutant C269A	Bacillus cereus
0.11	-	L-Ala	wild-tpye	Bacillus cereus
0.12	-	L-Ala	mutant C269A	Bacillus cereus

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Release 2023.1 (January 2023)