Literature summary for 6.2.1.52 extracted from

Viviani, V.; Scorsato, V.; Prado, R.; Pereira, J.; Niwa, K.; Ohmiya, Y.; Barbosa, J.
The origin of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase) Luciferin stereoselectivity as a switch for the oxygenase activity (2010), Photochem. Photobiol. Sci., 9, 1111-1119 .

Search for more literature for 6.2.1.52

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Zophobas atratus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.26	-	ATP	at pH 8.5, temperature not specified in the publication	Zophobas atratus
0.8	-	L-firefly luciferin	at pH 8.5, temperature not specified in the publication	Zophobas atratus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-firefly luciferin + CoA	Zophobas atratus	-	AMP + diphosphate + L-firefly luciferyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Zophobas atratus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
MiniQ anion exchange column chromatography, and Superdex 200 gel filtration	Zophobas atratus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-firefly luciferin + CoA	-	Zophobas atratus	AMP + diphosphate + L-firefly luciferyl-CoA	-	?

Subunits

Subunits	Comment	Organism
?	x * 57000, SDS-PAGE	Zophobas atratus

Synonyms

Synonyms	Comment	Organism
protoluciferase	-	Zophobas atratus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0015	-	ATP	at pH 8.5, temperature not specified in the publication	Zophobas atratus
0.0015	-	L-firefly luciferin	at pH 8.5, temperature not specified in the publication	Zophobas atratus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Zophobas atratus

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Release 2023.1 (January 2023)