BRENDA - Enzyme Database show
show all sequences of 6.2.1.52

Firefly luciferase exhibits bimodal action depending on the luciferin chirality

Nakamura, M.; Maki, S.; Amano, Y.; Ohkita, Y.; Niwa, K.; Hirano, T.; Ohmiya, Y.; Niwa, H.; Biochem. Biophys. Res. Commun. 331, 471-475 (2005)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-luciferin
-
Photinus pyralis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0982
-
L-firefly luciferin
at 25°C, pH not specified in the publication
Photinus pyralis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-firefly luciferin + CoA
Photinus pyralis
-
AMP + diphosphate + L-firefly luciferyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Photinus pyralis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-firefly luciferin + CoA
-
744214
Photinus pyralis
AMP + diphosphate + L-firefly luciferyl-CoA
-
-
-
?
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.135
-
at 25°C, pH not specified in the publication
Photinus pyralis
D-luciferin
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.135
-
at 25°C, pH not specified in the publication
Photinus pyralis
D-luciferin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-luciferin
-
Photinus pyralis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0982
-
L-firefly luciferin
at 25°C, pH not specified in the publication
Photinus pyralis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-firefly luciferin + CoA
Photinus pyralis
-
AMP + diphosphate + L-firefly luciferyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-firefly luciferin + CoA
-
744214
Photinus pyralis
AMP + diphosphate + L-firefly luciferyl-CoA
-
-
-
?
Other publictions for EC 6.2.1.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744417
Maeda
Biosynthesis-inspired deracem ...
Luciola cruciata
Biochim. Biophys. Acta
1861
2112-2118
2017
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-
1
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-
1
-
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1
-
4
-
-
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1
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1
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1
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1
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1
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-
-
-
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-
745978
Prado
Structural evolution of lucif ...
Zophobas atratus
Photochem. Photobiol. Sci.
10
1226-1232
2011
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-
-
-
1
-
-
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1
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4
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1
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1
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1
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1
-
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-
745606
Leitao
Firefly luciferase inhibition ...
Photinus pyralis
J. Photochem. Photobiol. B
101
1-8
2010
-
-
-
-
-
-
30
-
-
-
-
1
-
1
-
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1
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30
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1
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1
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-
745980
Viviani
-
The origin of luciferase acti ...
Zophobas atratus
Photochem. Photobiol. Sci.
9
1111-1119
2010
-
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
1
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-
1
1
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2
1
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1
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-
-
-
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-
2
-
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1
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1
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-
1
1
-
-
-
2
1
-
-
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-
-
-
-
-
-
745128
Marques
Firefly bioluminescence A mec ...
Photinus pyralis
IUBMB Life
61
6-17
2009
-
-
-
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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744868
Kato
New application of firefly lu ...
Aquatica lateralis
FEBS J.
274
3877-3885
2007
-
-
-
-
-
-
-
1
-
1
-
1
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5
-
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13
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1
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1
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1
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1
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13
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1
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744214
Nakamura
Firefly luciferase exhibits b ...
Photinus pyralis
Biochem. Biophys. Res. Commun.
331
471-475
2005
-
-
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1
1
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1
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1
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1
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1
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1
1
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1
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1
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1
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744710
Fraga
Identification of luciferyl a ...
Photinus pyralis
ChemBioChem
5
110-115
2004
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1
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1
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1
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1
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1
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