Protein Variants | Comment | Organism |
---|---|---|
W43F/W76F/W248F | mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme | Escherichia coli |
W76F | mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme | Escherichia coli |
General Stability | Organism |
---|---|
mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. ADP and ATP both protect the enzyme against inactivation by clostripain. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant W76F and W43,76,248F and wild type | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + succinate + CoA | - |
Escherichia coli | ADP + phosphate + succinyl-CoA | - |
? |