Literature summary for 6.2.1.5 extracted from

Nolte, J.C.; Schuermann, M.; Schepers, C.L.; Vogel, E.; Wuebbeler, J.H.; Steinbuechel, A.
Novel characteristics of succinate coenzyme A (succinate-CoA) ligases: conversion of malate to malyl-CoA and CoA-thioester formation of succinate analogues in vitro (2014), Appl. Environ. Microbiol., 80, 166-176.

Cloned(Commentary)

Cloned (Comment)	Organism
gene sucCD, expression in Escherichia coli strain BL21(DE3)-pLysS	Escherichia coli K-12
gene sucCD, expression in Escherichia coli strain BL21(DE3)-pLysS	Advenella mimigardefordensis
gene sucCD, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-pLysS	Alcanivorax borkumensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.004	-	CoA	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
0.037	-	CoA	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.055	-	ATP	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.058	-	CoA	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.141	-	succinate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.157	-	succinate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
0.182	-	succinate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.201	-	ATP	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.241	-	ATP	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
0.351	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.475	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
1.5	2	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
1.509	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
2.558	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
2.964	-	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
3.095	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
3.27	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
3.588	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
3.635	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
4.243	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + itaconate + CoA	Escherichia coli K-12	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	Advenella mimigardefordensis	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	Alcanivorax borkumensis	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	Alcanivorax borkumensis SK2	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	Advenella mimigardefordensis DPN7	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	Escherichia coli K-12 BL21	-	ADP + phosphate + itaconyl-CoA	-	r
ATP + succinate + CoA	Advenella mimigardefordensis	-	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	Alcanivorax borkumensis	-	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	Escherichia coli K-12	ATP is the preferred cosubstrate	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	Alcanivorax borkumensis SK2	-	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	Advenella mimigardefordensis DPN7	-	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	Escherichia coli K-12 BL21	ATP is the preferred cosubstrate	ADP + phosphate + succinyl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Advenella mimigardefordensis	W0PAN5	alpha-subunit; gene sucCD	-
Advenella mimigardefordensis	W0PFR9	beta-subunit; gene sucCD	-
Advenella mimigardefordensis DPN7	W0PAN5	alpha-subunit; gene sucCD	-
Advenella mimigardefordensis DPN7	W0PFR9	beta-subunit; gene sucCD	-
Alcanivorax borkumensis	Q0VPF7	beta-subunit; gene sucCD	-
Alcanivorax borkumensis	Q0VPF8	alpha-subunit; gene sucCD	-
Alcanivorax borkumensis SK2	Q0VPF7	beta-subunit; gene sucCD	-
Alcanivorax borkumensis SK2	Q0VPF8	alpha-subunit; gene sucCD	-
Escherichia coli K-12	P0A836	beta-subunit; gene sucCD	-
Escherichia coli K-12	P0AGE9	alpha-subunit; gene sucCD	-
Escherichia coli K-12 BL21	P0A836	beta-subunit; gene sucCD	-
Escherichia coli K-12 BL21	P0AGE9	alpha-subunit; gene sucCD	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His-tagged enzyme 1.55fold from Escherichia coli strain BL21(DE3)-pLysS by nickel affinity chromatography	Alcanivorax borkumensis
recombinant enzyme 1.07fold from Escherichia coli strain BL21(DE3)-pLysS by two different steps of anion exchange chromatography and affinity chromatography	Advenella mimigardefordensis
recombinant enzyme 1.47fold from Escherichia coli strain BL21(DE3)-pLysS by anion exchange and affinity chromatography	Escherichia coli K-12

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1	2	purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation	Advenella mimigardefordensis
4.14	-	purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation	Alcanivorax borkumensis
18.6	-	purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation	Escherichia coli K-12

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 3-sulfinopropionate + CoA	-	Escherichia coli K-12	ADP + phosphate + 3-sulfinopropionyl-CoA	-	r
ATP + 3-sulfinopropionate + CoA	-	Advenella mimigardefordensis	ADP + phosphate + 3-sulfinopropionyl-CoA	-	r
ATP + 3-sulfinopropionate + CoA	-	Advenella mimigardefordensis DPN7	ADP + phosphate + 3-sulfinopropionyl-CoA	-	r
ATP + 3-sulfinopropionate + CoA	-	Escherichia coli K-12 BL21	ADP + phosphate + 3-sulfinopropionyl-CoA	-	r
ATP + D-malate + CoA	-	Escherichia coli K-12	ADP + phosphate + D-malyl-CoA	-	r
ATP + D-malate + CoA	-	Advenella mimigardefordensis	ADP + phosphate + D-malyl-CoA	-	r
ATP + D-malate + CoA	-	Alcanivorax borkumensis	ADP + phosphate + D-malyl-CoA	-	r
ATP + D-malate + CoA	-	Alcanivorax borkumensis SK2	ADP + phosphate + D-malyl-CoA	-	r
ATP + D-malate + CoA	-	Advenella mimigardefordensis DPN7	ADP + phosphate + D-malyl-CoA	-	r
ATP + itaconate + CoA	-	Escherichia coli K-12	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	-	Advenella mimigardefordensis	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	-	Alcanivorax borkumensis	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	-	Alcanivorax borkumensis SK2	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	-	Advenella mimigardefordensis DPN7	ADP + phosphate + itaconyl-CoA	-	r
ATP + itaconate + CoA	-	Escherichia coli K-12 BL21	ADP + phosphate + itaconyl-CoA	-	r
ATP + L-malate + CoA	-	Escherichia coli K-12	ADP + phosphate + L-malyl-CoA	-	r
ATP + L-malate + CoA	-	Advenella mimigardefordensis	ADP + phosphate + L-malyl-CoA	-	r
ATP + L-malate + CoA	-	Alcanivorax borkumensis	ADP + phosphate + L-malyl-CoA	-	r
ATP + L-malate + CoA	-	Alcanivorax borkumensis SK2	ADP + phosphate + L-malyl-CoA	-	r
ATP + succinate + CoA	-	Advenella mimigardefordensis	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	-	Alcanivorax borkumensis	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	ATP is the preferred cosubstrate	Escherichia coli K-12	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	-	Alcanivorax borkumensis SK2	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	-	Advenella mimigardefordensis DPN7	ADP + phosphate + succinyl-CoA	-	r
ATP + succinate + CoA	ATP is the preferred cosubstrate	Escherichia coli K-12 BL21	ADP + phosphate + succinyl-CoA	-	r
GTP + succinate + CoA	-	Escherichia coli K-12	GDP + phosphate + succinyl-CoA	-	r
GTP + succinate + CoA	-	Escherichia coli K-12 BL21	GDP + phosphate + succinyl-CoA	-	r
additional information	substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, 3-sulfinopropionate, fumarate, maleate, and 2,2'-thiodiacetate	Advenella mimigardefordensis	?	-	?
additional information	substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Alcanivorax borkumensis	?	-	?
additional information	the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4, substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Escherichia coli K-12	?	-	?
additional information	the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4. substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Escherichia coli K-12	?	-	?
additional information	substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Alcanivorax borkumensis SK2	?	-	?
additional information	substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, 3-sulfinopropionate, fumarate, maleate, and 2,2'-thiodiacetate	Advenella mimigardefordensis DPN7	?	-	?
additional information	the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4. substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Escherichia coli K-12 BL21	?	-	?
additional information	the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4, substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate	Escherichia coli K-12 BL21	?	-	?

Synonyms

Synonyms	Comment	Organism
SucCD	-	Escherichia coli K-12
SucCD	-	Advenella mimigardefordensis
SucCD	-	Alcanivorax borkumensis
succinate coenzyme A ligase	-	Escherichia coli K-12
succinate coenzyme A ligase	-	Advenella mimigardefordensis
succinate coenzyme A ligase	-	Alcanivorax borkumensis
succinate-CoA ligase	-	Escherichia coli K-12
succinate-CoA ligase	-	Advenella mimigardefordensis
succinate-CoA ligase	-	Alcanivorax borkumensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Escherichia coli K-12
30	-	assay at	Advenella mimigardefordensis
30	-	assay at	Alcanivorax borkumensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.2	-	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.2	-	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.5	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
1.1	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
1.2	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
1.5	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
1.6	-	CoA	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
1.7	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
1.8	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
2.1	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
2.6	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
2.7	-	succinate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
3.6	-	ATP	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
5.3	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
14.3	-	succinate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
19.6	-	ATP	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
26.7	-	CoA	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
31.1	-	succinate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
40.3	-	CoA	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
58.8	-	ATP	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Escherichia coli K-12
7.4	-	assay at	Advenella mimigardefordensis
7.4	-	assay at	Alcanivorax borkumensis

Cofactor

Cofactor	Comment	Organism
ADP	-	Escherichia coli K-12
ADP	-	Advenella mimigardefordensis
ADP	-	Alcanivorax borkumensis
ATP	-	Escherichia coli K-12
ATP	-	Advenella mimigardefordensis
ATP	-	Alcanivorax borkumensis
GDP	-	Escherichia coli K-12
GTP	-	Escherichia coli K-12

General Information

General Information	Comment	Organism
evolution	malate-CoA ligases and succinate-CoA ligases might have the same evolutionary origin	Escherichia coli K-12

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.1	-	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.3	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
0.3	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.4	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
0.6	-	D-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
0.7	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
0.8	-	L-malate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
1	-	3-sulfinopropionate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
3.1	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
3.3	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
14.8	-	ATP	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
15.1	-	Itaconate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
17.2	-	succinate	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
101.4	-	succinate	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
171.1	-	succinate	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
292.5	-	ATP	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis
354.4	-	ATP	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
372.3	-	CoA	recombinant enzyme, pH 7.4, 30°C	Alcanivorax borkumensis
461.3	-	CoA	recombinant enzyme, pH 7.4, 30°C	Escherichia coli K-12
1100	-	CoA	recombinant enzyme, pH 7.4, 30°C	Advenella mimigardefordensis