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Literature summary for 6.2.1.45 extracted from

  • Chang, T.; Huang, Y.; Ou, Y.
    The role of ginsenosides in inhibiting ubiquitin activating enzyme (E1) activity (2014), J. Funct. Foods, 7, 462-470.
No PubMed abstract available
Search for more literature for 6.2.1.45

Activating Compound

Activating Compound Comment Organism Structure
ginsenoside Rb1 increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
ginsenoside Rb2 increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
ginsenoside Rc increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
ginsenoside Rd increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens

Application

Application Comment Organism
medicine functional foods, e.g. ginsenoside Rg1 in Panax ginseng root, targeting the ubiquitin-activating enzyme (E1) of the ubiquitin-proteasome pathway as anti-tumour agents are considered to be important for cancer chemoprevention Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of mRFP-enzyme in Escherichia coli strain DH5alpha, linking of ubiquitin to the C-terminus of RFP through a peptide bond Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ginsenoside Re inhibits ubiquitin-activating enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
ginsenoside Rg1 inhibit ubiquitin-activating enzyme, from Panax ginseng roots, a traditional herbal medicine or food, causes 89.2% inhibition at 0.05 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, recombinant mRFP-enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Homo sapiens E1-activating enzyme activates ubiquitin via an adenylated intermediate and catalyzes its transfer to an E2 enzyme AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 eight human E1s
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mRFP-enzyme from Escherichia coli strain DH5alpha by combined anion exchange/affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine E1-activating enzyme activates ubiquitin via an adenylated intermediate and catalyzes its transfer to an E2 enzyme Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine E1 activates ubiquitin or an ubiquitin-like protein and transfers it to the E2-conjugating enzyme Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, recombinant mRFP-enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
E1
-
 Homo sapiens
ubiquitin activating enzyme
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0035
-
 recombinant enzyme, pH 7.6, 37°C Homo sapiens ginsenoside Rg1
0.0628
-
 recombinant enzyme, pH 7.6, 37°C Homo sapiens ginsenoside Re