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Literature summary for 6.2.1.45 extracted from

  • Miao, H.X.; Qin, Y.H.; Ye, Z.X.; Hu, G.B.
    Molecular characterization and expression analysis of ubiquitin-activating enzyme E1 gene in Citrus reticulata (2013), Gene, 513, 249-259.
    View publication on PubMed
Search for more literature for 6.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
gene CrUBE1, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, semi-quantitative RT-PCR and quantitative real-time PCR enzyme expression anayses, recombinant expression in Pichia pastoris strain GS115 Citrus reticulata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Citrus reticulata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49280
-
 x * 49280, sequence calculation Citrus reticulata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Citrus reticulata
-
 AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Citrus reticulata
-
 cv. Wuzishatangju (seedless) and cv. Shatangju (seedy), single copy gene CrUBE1
-

Source Tissue

Source Tissue Comment Organism Textmining
anther expression level of the CrUBE1 gene in anthers of cv. Shatangju is approximately 10fold higher than in anthers of cv. Wuzishatangju Citrus reticulata
-
carpel high enzyme expression level Citrus reticulata
-
filament very low expression Citrus reticulata
-
flower bud
-
 Citrus reticulata
-
leaf very low expression Citrus reticulata
-
leaf bud
-
 Citrus reticulata
-
additional information semi-quantitative and quantitative expressio analysis of the enzyme in different tissues of the two cultivars, o verview, the highest expression level of CrUBE1 is detected in pistils at 7 days after self pollination of cv.Wuzishatangju, which is approximately 5fold higher than at the starting point Citrus reticulata
-
petal very low expression Citrus reticulata
-
plant ovary
-
 Citrus reticulata
-
stigma
-
 Citrus reticulata
-
style very low expression Citrus reticulata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
-
 Citrus reticulata AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 49280, sequence calculation Citrus reticulata

Synonyms

Synonyms Comment Organism
UBE1
-
 Citrus reticulata
ubiquitin-activating enzyme E1
-
 Citrus reticulata

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Citrus reticulata

pI Value

Organism Comment pI Value Maximum pI Value
Citrus reticulata sequence calculation
-
 5.44

Expression

Organism Comment Expression
Citrus reticulata self pollination induces the enzyme in cv. Wuzishatangju up

General Information

General Information Comment Organism
evolution 5 amino acids and 8 bases are different in cDNA and DNA sequences of CrUBE1 between Wuzishatangju and Shatangju, respectively Citrus reticulata
metabolism ubiquitin-activating enzyme E1 catalyzes the first step in the ubiquitination reaction, which targets a protein for degradation via a proteasome pathway. The enzyme plays an important role in metabolic processes Citrus reticulata
additional information the enzyme has six ATP binding sites, one catalytic residue, and one E2 binding domain Citrus reticulata
physiological function UBE1s catalyze the first steps in the ubiquitin conjugation cascade and are potentially involved in compatible pollination and ubiquitinmediated S-RNase degradation Citrus reticulata