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Literature summary for 6.2.1.45 extracted from

  • Wee, K.E.; Lai, Z.; Auger, K.R.; Ma, J.; Horiuchi, K.Y.; Dowling, R.L.; Dougherty, C.S.; Corman, J.I.; Wynn, R.; Copeland, R.A.
    Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate (2000), J. Protein Chem., 19, 489-498.
    View publication on PubMed
Search for more literature for 6.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expression in baculoviral system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
[ubiquitin carrier protein Ubc4]-L-cysteine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
 ubiquitin pH 7.5, 25°C Homo sapiens
0.00017
-
 Oregon Green-labeled ubiquitin pH 7.5, 25°C Homo sapiens
0.0047
-
 ATP pH 7.5, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
 analytical ultracentrifugation Homo sapiens
117300
-
 1 * 117300, calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Oregon Green-labeled ubiquitin + [ubiquitin-activating protein E1]-L-cysteine enzyme efficiently accepts ubiquitin substrate fluorescently labeled by Oregon Green Homo sapiens AMP + diphosphate + [ubiquitin-activating protein E1]-S-(Oregon Green-labeled ubiquitinyl)-L-cysteine
-
 ?
ATP + ubiquitin + [ubiquitin-activating protein E1]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 117300, calculated Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0035
-
 [ubiquitin carrier protein Ubc4]-L-cysteine pH 7.5, 25°C Homo sapiens