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Literature summary for 6.2.1.45 extracted from

  • Hatfield, P.M.; Gosink, M.M.; Carpenter, T.B.; Vierstra, R.D.
    The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana (1997), Plant J., 11, 213-226.
    View publication on PubMed
Search for more literature for 6.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 x * 12000 and x * 124000, SDS-PAGE, x * 119600, calculated Arabidopsis thaliana
119600
-
 x * 12000 and x * 124000, SDS-PAGE, x * 119600, calculated Arabidopsis thaliana
120300
-
 x * 123000, SDS-PAGE, x * 120300, calculated Arabidopsis thaliana
123000
-
 x * 123000, SDS-PAGE, x * 120300, calculated Arabidopsis thaliana
124000
-
 x * 12000 and x * 124000, SDS-PAGE, x * 119600, calculated Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P92974 isoform UBA2
-
Arabidopsis thaliana P93028 isoform UBA1
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ubiquitin + [ubiquitin-activating protein UBA1]-L-cysteine enzyme forms higher molecular mass intermediates with ubiquitin Arabidopsis thaliana AMP + diphosphate + [ubiquitin-activating protein UBA1]-S-ubiquitinyl-L-cysteine the enzyme-ubiquitin intermediates dissociate in presence of 2-mercaptoethanol, indicating thiolester linkage ?
ATP + ubiquitin + [ubiquitin-activating protein UBA2]-L-cysteine enzyme forms higher molecular mass intermediates with ubiquitin Arabidopsis thaliana AMP + diphosphate + [ubiquitin-activating protein UBA2]-S-ubiquitinyl-L-cysteine the enzyme-ubiquitin intermediates dissociate in presence of 2-mercaptoethanol, indicating thiolester linkage ?

Subunits

Subunits Comment Organism
? x * 123000, SDS-PAGE, x * 120300, calculated Arabidopsis thaliana
? x * 12000 and x * 124000, SDS-PAGE, x * 119600, calculated Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Uba1
-
 Arabidopsis thaliana
UBA2
-
 Arabidopsis thaliana