BRENDA - Enzyme Database show
show all sequences of 6.2.1.44

Novel pathway for assimilation of dimethylsulphoniopropionate widespread in marine bacteria

Reisch, C.R.; Stoudemayer, M.J.; Varaljay, V.A.; Amster, I.J.; Moran, M.A.; Whitman, W.B.; Nature 473, 208-211 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the dmdB gene is cloned into the pET101 expression vector and expressed in Escherichia coli, and the recombinant Escherichia coli strain possesses 3-(methylthio)propionyl-CoA ligase activity, whereas the host strain alone does not
Ruegeria pomeroyi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59066
-
x * 59066, calculated from sequence
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ruegeria pomeroyi
Q5LRT0
-
-
Purification (Commentary)
Commentary
Organism
-
Ruegeria pomeroyi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.118
-
chemostat, pH and temperature not specified in the publication
Ruegeria pomeroyi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-(methylthio)propanoate + CoA
-
728373
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
ATP + 3-(methylthio)propanoate + CoA
degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol
728373
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 59066, calculated from sequence
Ruegeria pomeroyi
Cloned(Commentary) (protein specific)
Commentary
Organism
the dmdB gene is cloned into the pET101 expression vector and expressed in Escherichia coli, and the recombinant Escherichia coli strain possesses 3-(methylthio)propionyl-CoA ligase activity, whereas the host strain alone does not
Ruegeria pomeroyi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59066
-
x * 59066, calculated from sequence
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ruegeria pomeroyi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.118
-
chemostat, pH and temperature not specified in the publication
Ruegeria pomeroyi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-(methylthio)propanoate + CoA
-
728373
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
ATP + 3-(methylthio)propanoate + CoA
degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol
728373
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 59066, calculated from sequence
Ruegeria pomeroyi
Expression
Organism
Commentary
Expression
Ruegeria pomeroyi
the amount of transcripts for dmdB, dmdC and dmdD increases during growth of wild-type enzyme on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism
up
General Information
General Information
Commentary
Organism
malfunction
growth of a dmdB mutant (SPO2045::tet) is somewhat delayed during growth on 3-(methylthio)propionate. Following growth with 3-(dimethylsulphonio)propanoate, the DmdB activity is reduced by only 40% compared to the wild-type
Ruegeria pomeroyi
metabolism
in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min* mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increased during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for methylmercaptopropionate metabolism
Ruegeria pomeroyi
General Information (protein specific)
General Information
Commentary
Organism
malfunction
growth of a dmdB mutant (SPO2045::tet) is somewhat delayed during growth on 3-(methylthio)propionate. Following growth with 3-(dimethylsulphonio)propanoate, the DmdB activity is reduced by only 40% compared to the wild-type
Ruegeria pomeroyi
metabolism
in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min* mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increased during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for methylmercaptopropionate metabolism
Ruegeria pomeroyi
Expression (protein specific)
Organism
Commentary
Expression
Ruegeria pomeroyi
the amount of transcripts for dmdB, dmdC and dmdD increases during growth of wild-type enzyme on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism
up
Other publictions for EC 6.2.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727780
Bullock
Regulatory and functional dive ...
Burkholderia thailandensis, Burkholderia thailandensis ATCC 700388, Candidatus Pelagibacter ubique, Candidatus Pelagibacter ubique HTCC1062, Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692, Ruegeria lacuscaerulensis, Ruegeria lacuscaerulensis ITI-1157, Ruegeria pomeroyi
J. Bacteriol.
196
1275-1285
2014
-
-
5
-
-
-
41
12
-
14
13
3
-
25
-
-
5
-
-
-
3
3
109
5
2
2
-
12
3
-
-
-
-
-
-
-
-
7
-
-
-
-
-
54
-
12
-
17
13
3
-
-
-
7
-
-
3
3
109
7
3
3
-
12
3
-
-
-
1
1
2
2
12
12
728373
Reisch
Novel pathway for assimilation ...
Ruegeria pomeroyi
Nature
473
208-211
2011
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-