Cloned (Comment) | Organism |
---|---|
the dmdB gene is cloned into the pET101 expression vector and expressed in Escherichia coli, and the recombinant Escherichia coli strain possesses 3-(methylthio)propionyl-CoA ligase activity, whereas the host strain alone does not | Ruegeria pomeroyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59066 | - |
x * 59066, calculated from sequence | Ruegeria pomeroyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-(methylthio)propanoate + CoA | Ruegeria pomeroyi | degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol | AMP + diphosphate + 3-(methylthio)propionyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruegeria pomeroyi | Q5LRT0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruegeria pomeroyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.118 | - |
chemostat, pH and temperature not specified in the publication | Ruegeria pomeroyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-(methylthio)propanoate + CoA | - |
Ruegeria pomeroyi | AMP + diphosphate + 3-(methylthio)propionyl-CoA | - |
? | |
ATP + 3-(methylthio)propanoate + CoA | degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol | Ruegeria pomeroyi | AMP + diphosphate + 3-(methylthio)propionyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59066, calculated from sequence | Ruegeria pomeroyi |
Synonyms | Comment | Organism |
---|---|---|
DmdB | - |
Ruegeria pomeroyi |
MMPA-CoA ligase | - |
Ruegeria pomeroyi |
SPO2045 | - |
Ruegeria pomeroyi |
Organism | Comment | Expression |
---|---|---|
Ruegeria pomeroyi | the amount of transcripts for dmdB, dmdC and dmdD increases during growth of wild-type enzyme on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism | up |
General Information | Comment | Organism |
---|---|---|
malfunction | growth of a dmdB mutant (SPO2045::tet) is somewhat delayed during growth on 3-(methylthio)propionate. Following growth with 3-(dimethylsulphonio)propanoate, the DmdB activity is reduced by only 40% compared to the wild-type | Ruegeria pomeroyi |
metabolism | in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min* mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increased during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for methylmercaptopropionate metabolism | Ruegeria pomeroyi |