BRENDA - Enzyme Database
show all sequences of 6.2.1.41

FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria

Casabon, I.; Crowe, A.M.; Liu, J.; Eltis, L.D.; Mol. Microbiol. 87, 269-283 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
neither 1beta(2'-propanoate)-3aalpha-H-4a(3''-propanoate)-7alphabeta-methylhexahydro-5-indanone nor 3aalpha-H-4aalpha(3'-propanoic acid)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone significantly inhibit enzyme activity
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0098
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
0.11
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
3
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
Mycobacterium tuberculosis
-
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-Sepharose 6 column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
-
730411
Mycobacterium tuberculosis
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
-
?
ATP + 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
-
730411
Mycobacterium tuberculosis
?
-
-
-
?
additional information
no activity with 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone, 3-oxo-23,24-bisnorchol-4-en-22-oate, cholate, and 3beta-hydroxy-5-cholesten-26-oate
730411
Mycobacterium tuberculosis
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
fadD3
-
Mycobacterium tuberculosis
HIP-CoA ligase
-
Mycobacterium tuberculosis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.1
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
7.5
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
13
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
neither 1beta(2'-propanoate)-3aalpha-H-4a(3''-propanoate)-7alphabeta-methylhexahydro-5-indanone nor 3aalpha-H-4aalpha(3'-propanoic acid)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone significantly inhibit enzyme activity
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0098
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
0.11
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
3
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
Mycobacterium tuberculosis
-
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-Sepharose 6 column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
-
730411
Mycobacterium tuberculosis
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
-
?
ATP + 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
-
730411
Mycobacterium tuberculosis
?
-
-
-
?
additional information
no activity with 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone, 3-oxo-23,24-bisnorchol-4-en-22-oate, cholate, and 3beta-hydroxy-5-cholesten-26-oate
730411
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.1
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
7.5
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
13
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
66
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
730
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22°C
Mycobacterium tuberculosis
66
-
CoA
at pH 7.3 and 22°C
Mycobacterium tuberculosis
730
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22°C
Mycobacterium tuberculosis
Other publictions for EC 6.2.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730411
Casabon
FadD3 is an acyl-CoA synthetas ...
Mycobacterium tuberculosis
Mol. Microbiol.
87
269-283
2013
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