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Literature summary for 6.2.1.41 extracted from
- FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria (2013), Mol. Microbiol., 87, 269-283.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3)pLysS cells | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neither 1beta(2'-propanoate)-3aalpha-H-4a(3''-propanoate)-7alphabeta-methylhexahydro-5-indanone nor 3aalpha-H-4aalpha(3'-propanoic acid)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone significantly inhibit enzyme activity | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0098 | - |
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate | at pH 7.3 and 22°C | Mycobacterium tuberculosis |  |
| 0.11 | - |
CoA | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
| 3 | - |
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA | Mycobacterium tuberculosis | - |
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose 6 column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA | - |
Mycobacterium tuberculosis | AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA | - |
? | |
| ATP + 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone | - |
Mycobacterium tuberculosis | ? | - |
? | |
| additional information | no activity with 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone, 3-oxo-23,24-bisnorchol-4-en-22-oate, cholate, and 3beta-hydroxy-5-cholesten-26-oate | Mycobacterium tuberculosis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fadD3 | - |
Mycobacterium tuberculosis |
| HIP-CoA ligase | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.1 | - |
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
| 7.5 | - |
CoA | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
| 13 | - |
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
| 66 | - |
CoA | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
| 730 | - |
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate | at pH 7.3 and 22°C | Mycobacterium tuberculosis | |
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