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Literature summary for 6.2.1.4 extracted from

  • Schuermann, M.; Wuebbeler, J.H.; Grote, J.; Steinbuechel, A.
    Novel reaction of succinyl coenzyme A (succinyl-CoA) synthetase: activation of 3-sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis strain DPN7T during degradation of 3,3-dithiodipropionic acid (2011), J. Bacteriol., 193, 3078-3089.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene sucCD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pLysS Advenella mimigardefordensis
gene sucCD, expression in Escherichia coli strain BL21(DE3)/pLysS Advenella mimigardefordensis

Protein Variants

Protein Variants Comment Organism
additional information transposon mutagenesis and deletion of gene sucCD, accumulation of 3SP during 3,3'-dithiodipropionic acid degradation occurs in Tn5::mob-induced mutants of Advenella mimigardefordensis strain DPN7T disrupted in sucCD and in the defined deletion mutant Advenella mimigardefordensis DELTAsucCD Advenella mimigardefordensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis
Mn2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + succinate + CoA Advenella mimigardefordensis SucCDAm is unspecific regarding ATP or GTP GDP + phosphate + succinyl-CoA
-
 ?
GTP + succinate + CoA Advenella mimigardefordensis DPN7T SucCDAm is unspecific regarding ATP or GTP GDP + phosphate + succinyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Advenella mimigardefordensis B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
Advenella mimigardefordensis B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
Advenella mimigardefordensis DPN7T B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
Advenella mimigardefordensis DPN7T B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli strain BL21(DE3)/pLysS by anion exchange chromatography Advenella mimigardefordensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + 3-sulfinopropionate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + 3-sulfinopropionyl-CoA determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry ?
GTP + 3-sulfinopropionate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + 3-sulfinopropionyl-CoA determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry ?
GTP + itaconate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + itaconyl-CoA
-
 ?
GTP + itaconate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + itaconyl-CoA
-
 ?
GTP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + succinyl-CoA
-
 ?
GTP + succinate + CoA succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + succinyl-CoA
-
 ?
GTP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + succinyl-CoA
-
 ?
GTP + succinate + CoA succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + succinyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
SucCDAm
-
 Advenella mimigardefordensis
Succinyl coenzyme A synthetase
-
 Advenella mimigardefordensis
Succinyl-CoA synthetase
-
 Advenella mimigardefordensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Advenella mimigardefordensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Advenella mimigardefordensis

Cofactor

Cofactor Comment Organism Structure
GTP SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis