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Literature summary for 6.2.1.39 extracted from
- Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway (2005), Chem. Biol., 12, 665-675.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene btrJ, butirosin genetic cluster organization, overview. Expression of N-terminal His6-tagged BtrJ | Niallia circulans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essentially required | Niallia circulans |  |
| Mn2+ | essentially required, activates | Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48600 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
| 50962 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
| 50969 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | Niallia circulans | - |
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | Niallia circulans NR3312 | - |
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + BtrI acyl-carrier protein | Niallia circulans | - |
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + BtrI acyl-carrier protein | Niallia circulans NR3312 | - |
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q4H4E7 | gene btrJ | - |
| Niallia circulans NR3312 | Q4H4E7 | gene btrJ | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-aminobutanoate + BtrI acyl-carrier protein | - |
Niallia circulans | ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein] | GABA-S-BtrI is closely related to holo-BtrI | ? | |
| ATP + 4-aminobutanoate + BtrI acyl-carrier protein | - |
Niallia circulans NR3312 | ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein] | GABA-S-BtrI is closely related to holo-BtrI | ? | |
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | - |
Niallia circulans | ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | - |
Niallia circulans NR3312 | ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond | Niallia circulans | ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond | Niallia circulans NR3312 | ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + BtrI acyl-carrier protein | - |
Niallia circulans | ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
| ATP + L-glutamate + BtrI acyl-carrier protein | - |
Niallia circulans NR3312 | ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
| additional information | the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay | Niallia circulans | ? | - |
? | |
| additional information | the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay | Niallia circulans NR3312 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
| More | purified BtrJ shows aggregation in solution, preventable by DTT | Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BtrJ | - |
Niallia circulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Niallia circulans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | BtrJ belongs to the ATP-grasp superfamily | Niallia circulans |
| metabolism | butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview | Niallia circulans |
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