Camphor revisited: Involvement of a unique monooxygenase in metabolism of 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida
Ougham, H.; Taylor, D.; Trudgill, P.; J. Bacteriol. 153, 140-152 (1983) 
Data extracted from this reference:
Engineering
Protein Variants
Commentary
Organism
additional information
the ability to grow on (+)-camphor is transferred to a recipient strain, Pseudomonas putida PaW340, which is a streptomycin-resistant tryptophan auxotroph that is derived from Pseudomonas putida (arvilla) mt-2
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
Pseudomonas putida
-
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
Pseudomonas putida C1 / ATCC 17453
-
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
no activity in Pseudomonas putida strain PaW340
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida C1 / ATCC 17453
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:(+)-camphor-grown cell
inducible enzyme, no or poor activity in succinate- and octanoate-grown cells
Pseudomonas putida
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
717711
Pseudomonas putida
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
-
?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
717711
Pseudomonas putida C1 / ATCC 17453
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2-oxo-3-4,5,5-trimethylcyclopentenylacetyl CoA synthetase
-
Pseudomonas putida
2-oxo-3-4,5,5-trimethylcyclopentenylacetyl coenzyme A synthetase
-
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
the ability to grow on (+)-camphor is transferred to a recipient strain, Pseudomonas putida PaW340, which is a streptomycin-resistant tryptophan auxotroph that is derived from Pseudomonas putida (arvilla) mt-2
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
Pseudomonas putida
-
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
Pseudomonas putida C1 / ATCC 17453
-
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:(+)-camphor-grown cell
inducible enzyme, no or poor activity in succinate- and octanoate-grown cells
Pseudomonas putida
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
717711
Pseudomonas putida
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
-
?
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA
-
717711
Pseudomonas putida C1 / ATCC 17453
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas putida
Expression
Organism
Commentary
Expression
Pseudomonas putida
the enzyme is induced by cell growth on (+)-camphor as carbon source
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the (+)-camphor catabolism. Metabolism of the first ring cleavage intermediate, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, occurs through the sequential action of two inducible enzymes, the coenzyme A ester synthetase and an oxygenase, EC 1.14.13.160
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the (+)-camphor catabolism. Metabolism of the first ring cleavage intermediate, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, occurs through the sequential action of two inducible enzymes, the coenzyme A ester synthetase and an oxygenase, EC 1.14.13.160
Pseudomonas putida
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas putida
the enzyme is induced by cell growth on (+)-camphor as carbon source
up
Other publictions for EC 6.2.1.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717711
Ougham
Camphor revisited: Involvement ...
Pseudomonas putida, no activity in Pseudomonas putida strain PaW340, Pseudomonas putida C1 / ATCC 17453
J. Bacteriol.
153
140-152
1983
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