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Literature summary for 6.2.1.37 extracted from
- 3-Hydroxybenzoate:coenzyme A ligase from cell cultures of Centaurium erythraea: isolation and characterization (2000), Biol. Chem., 381, 155-160.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 5% does not affect enzyme stability | Centaurium erythraea |
| the enzyme could be stored frozen for several months without appreciable loss of activity | Centaurium erythraea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
CoA | pH 7.5, 35°C | Centaurium erythraea |  |
| 0.0147 | - |
3-hydroxybenzoate | pH 7.5, 35°C | Centaurium erythraea | |
| 0.229 | - |
ATP | pH 7.5, 35°C | Centaurium erythraea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strictly dependent on Mg2+ or Mn2+ ions, with concentrations of 0.5 mM leading to maximum activity. No enzyme activity was found in the presence of Ca2+ | Centaurium erythraea | |
| Mn2+ | strictly dependent on Mg2+ or Mn2+ ions, with concentrations of 0.5 mM leading to maximum activity. No enzyme activity was found in the presence of Ca2+ | Centaurium erythraea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Centaurium erythraea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxybenzoate + CoA | Centaurium erythraea | the enzyme is involved in the biosynthesis of xanthones | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | 5%, does not affect enzyme stability | Centaurium erythraea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Centaurium erythraea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Centaurium erythraea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Centaurium erythraea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crude extracts prepared with 0.1 M potassium phosphate buffer, pH 7.5, complete loss of activity within 24 h | Centaurium erythraea |
| the enzyme could be stored frozen for several months without appreciable loss of activity | Centaurium erythraea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxybenzoate + CoA | - |
Centaurium erythraea | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
| ATP + 3-hydroxybenzoate + CoA | the enzyme is involved in the biosynthesis of xanthones | Centaurium erythraea | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | two polypeptides of 41500 Da and 40500 Da are detected by SDS-PAGE, difference in their apparent molecular masses can not be attributed to glycosylation | Centaurium erythraea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3HBL | - |
Centaurium erythraea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Centaurium erythraea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Centaurium erythraea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity | Centaurium erythraea |
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