Protein Variants | Comment | Organism |
---|---|---|
E306Q | mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate | Pseudomonas sp. |
G163I | mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate | Pseudomonas sp. |
G166I | mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate | Pseudomonas sp. |
K169M | mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate | Pseudomonas sp. |
P168A | mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
4-Chlorobenzoate | wild-type | Pseudomonas sp. | |
0.02 | - |
4-Chlorobenzoate | mutant K169M | Pseudomonas sp. | |
0.062 | - |
CoA | mutant G163I | Pseudomonas sp. | |
0.073 | - |
4-Chlorobenzoate | mutant G166I | Pseudomonas sp. | |
0.076 | - |
CoA | mutant K169M | Pseudomonas sp. | |
0.085 | - |
CoA | wild-type | Pseudomonas sp. | |
0.105 | - |
4-Chlorobenzoate | mutant G163I | Pseudomonas sp. | |
0.108 | - |
CoA | mutant G166I | Pseudomonas sp. | |
0.168 | - |
CoA | mutant E306Q | Pseudomonas sp. | |
0.253 | - |
4-Chlorobenzoate | mutant E306Q | Pseudomonas sp. | |
0.308 | - |
ATP | wild-type | Pseudomonas sp. | |
0.321 | - |
ATP | mutant E306Q | Pseudomonas sp. | |
0.83 | - |
ATP | mutant K169M | Pseudomonas sp. | |
0.981 | - |
ATP | mutant G166I | Pseudomonas sp. | |
1.314 | - |
ATP | mutant G163I | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | - |
wild-type and mutant enzymes G163I, G166I, P168A, K169M and E306Q | - |
Pseudomonas sp. CBS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate | two-step reaction consisting of the adenylation of 4-chlorobenzoate with adenosine 5'-triphosphate followed by acyl transfer from 4-chlorobenzoyl adenosine 5'-monophosphate diester | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Chlorobenzoate + CoA + ATP | - |
Pseudomonas sp. | 4-Chlorobenzoyl-CoA + AMP + diphosphate | - |
? | |
4-Chlorobenzoate + CoA + ATP | - |
Pseudomonas sp. CBS3 | 4-Chlorobenzoyl-CoA + AMP + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
4-Chlorobenzoate | mutant G163I | Pseudomonas sp. | |
0.039 | - |
ATP | mutant G163I | Pseudomonas sp. | |
0.04 | - |
CoA | mutant G163I | Pseudomonas sp. | |
0.7 | - |
4-Chlorobenzoate | mutant E306Q | Pseudomonas sp. | |
0.8 | - |
ATP | mutant E306Q | Pseudomonas sp. | |
0.9 | - |
ATP | mutant E306Q | Pseudomonas sp. | |
2.3 | - |
4-Chlorobenzoate | mutant G166I | Pseudomonas sp. | |
2.7 | - |
CoA | mutant G166I | Pseudomonas sp. | |
3.3 | - |
ATP | mutant G166I | Pseudomonas sp. | |
9.4 | - |
ATP | mutant K169M | Pseudomonas sp. | |
9.8 | - |
4-Chlorobenzoate | mutant K169M | Pseudomonas sp. | |
16 | - |
ATP | mutant K169M | Pseudomonas sp. | |
34 | - |
4-Chlorobenzoate | wild-type | Pseudomonas sp. | |
39 | - |
CoA | wild-type | Pseudomonas sp. | |
54 | - |
ATP | wild-type | Pseudomonas sp. |