Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.33 extracted from

  • Chang, K.H.; Xiang, H.; Dunaway-Mariano, D.
    Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily: a site-directed mutagenesis study with the Pseudomonas sp. strain CBS3 4-chlorobenzoate:coenzyme A ligase (1997), Biochemistry, 36, 15650-15659.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E306Q mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
G163I mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
G166I mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
K169M mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.
P168A mutant enzymes G163I, G166I, P168A, K169M and E306Q with reduced catalysis of the adenylation of 4-chlorobenzoate Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
4-Chlorobenzoate wild-type Pseudomonas sp.
0.02
-
4-Chlorobenzoate mutant K169M Pseudomonas sp.
0.062
-
CoA mutant G163I Pseudomonas sp.
0.073
-
4-Chlorobenzoate mutant G166I Pseudomonas sp.
0.076
-
CoA mutant K169M Pseudomonas sp.
0.085
-
CoA wild-type Pseudomonas sp.
0.105
-
4-Chlorobenzoate mutant G163I Pseudomonas sp.
0.108
-
CoA mutant G166I Pseudomonas sp.
0.168
-
CoA mutant E306Q Pseudomonas sp.
0.253
-
4-Chlorobenzoate mutant E306Q Pseudomonas sp.
0.308
-
ATP wild-type Pseudomonas sp.
0.321
-
ATP mutant E306Q Pseudomonas sp.
0.83
-
ATP mutant K169M Pseudomonas sp.
0.981
-
ATP mutant G166I Pseudomonas sp.
1.314
-
ATP mutant G163I Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp.
-
wild-type and mutant enzymes G163I, G166I, P168A, K169M and E306Q
-
Pseudomonas sp. CBS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Reaction

Reaction Comment Organism Reaction ID
4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate two-step reaction consisting of the adenylation of 4-chlorobenzoate with adenosine 5'-triphosphate followed by acyl transfer from 4-chlorobenzoyl adenosine 5'-monophosphate diester Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Chlorobenzoate + CoA + ATP
-
Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
?
4-Chlorobenzoate + CoA + ATP
-
Pseudomonas sp. CBS3 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.036
-
4-Chlorobenzoate mutant G163I Pseudomonas sp.
0.039
-
ATP mutant G163I Pseudomonas sp.
0.04
-
CoA mutant G163I Pseudomonas sp.
0.7
-
4-Chlorobenzoate mutant E306Q Pseudomonas sp.
0.8
-
ATP mutant E306Q Pseudomonas sp.
0.9
-
ATP mutant E306Q Pseudomonas sp.
2.3
-
4-Chlorobenzoate mutant G166I Pseudomonas sp.
2.7
-
CoA mutant G166I Pseudomonas sp.
3.3
-
ATP mutant G166I Pseudomonas sp.
9.4
-
ATP mutant K169M Pseudomonas sp.
9.8
-
4-Chlorobenzoate mutant K169M Pseudomonas sp.
16
-
ATP mutant K169M Pseudomonas sp.
34
-
4-Chlorobenzoate wild-type Pseudomonas sp.
39
-
CoA wild-type Pseudomonas sp.
54
-
ATP wild-type Pseudomonas sp.