Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.33 extracted from

  • Wu, R.; Reger, A.S.; Lu, X.; Gulick, A.M.; Dunaway-Mariano, D.
    The mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-chlorobenzoate: coenzyme A ligase (2009), Biochemistry, 48, 4115-4125.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant D402P, to 2.6 A resolution, and comparison with wild-type. The C-terminal domain rotates by about 140 degrees between the two states that catalyze the adenylation and thioester-forming half-reactions. The domain rotation is accompanied by a change in the main chain torsional angles of residue D402 Alcaligenes sp. AL3007

Protein Variants

Protein Variants Comment Organism
D402A 100fold decrease in ratio kcat/Km for CoA Alcaligenes sp. AL3007
D402P crystallization data. Mutant adopts the proposed adenylate-forming conformation with very little change to the overall structure.The ability of the mutant to catalyze the adenylate-forming half-reaction is reduced by about 3fold, catalysis of the second half-reaction is reduced by 4 orders of magnitude Alcaligenes sp. AL3007

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
4-Chlorobenzoate wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
0.0012
-
4-Chlorobenzoate mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
0.0023
-
4-Chlorobenzoate mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
0.0131
-
ATP mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
0.0281
-
ATP mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
0.124
-
ATP wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
0.31
-
CoA wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
2
-
CoA mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
6
-
CoA mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. AL3007
-
-
-
Alcaligenes sp. AL3007 Q8GN86 mutant D402P
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Chlorobenzoate + CoA + ATP
-
Alcaligenes sp. AL3007 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
4-Chlorobenzoate mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
1
-
ATP mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
1.8
-
CoA mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
2.8
-
ATP mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
3.4
-
4-Chlorobenzoate mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
3.7
-
CoA mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
9.2
-
4-Chlorobenzoate wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
9.3
-
CoA wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
11.5
-
ATP wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
CoA mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
0.3
-
CoA mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
2
-
ATP mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
30
-
4-Chlorobenzoate mutant D402P, pH 7.5, 25°C Alcaligenes sp. AL3007
30
-
CoA wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
40
-
ATP mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
90
-
ATP wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007
400
-
4-Chlorobenzoate mutant D402A, pH 7.5, 25°C Alcaligenes sp. AL3007
10000
-
4-Chlorobenzoate wild-type, pH 7.5, 25°C Alcaligenes sp. AL3007