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Literature summary for 6.2.1.33 extracted from

  • Zhou, L.; Marks, T.S.; Poh, R.P.; Smith, R.J.; Chowdhry, B.Z.; Smith, A.R.
    The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1 (2004), Biodegradation, 15, 97-109.
    View publication on PubMed
Search for more literature for 6.2.1.33

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 4-Chlorobenzoate 25°C, pH 7.0 Arthrobacter sp.
0.0238
-
 ATP 25°C, pH 7.0 Arthrobacter sp.
0.03
-
 CoA 25°C, pH 7.0 Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE Arthrobacter sp.
112000
-
 gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. TM-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.86
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Bromobenzoate + CoA + ATP 93% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Bromobenzoate + CoA + ATP 93% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Chlorobenzoate + CoA + ATP
-
 Arthrobacter sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Chlorobenzoate + CoA + ATP
-
 Arthrobacter sp. TM-1 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Fluorobenzoate + CoA + ATP 67% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Fluorobenzoate + CoA + ATP 67% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Iodobenzoate + CoA + ATP 73% of the activity with 4-chlorobenzoate Arthrobacter sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Iodobenzoate + CoA + ATP 73% of the activity with 4-chlorobenzoate Arthrobacter sp. TM-1 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
Benzoate + CoA + ATP 62% of the activity with 4-chlorobenzoate Arthrobacter sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
4-CB:CoA ligase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 10°C: about 80% of maximal activity, 40°C: about 50% of maximal activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.2 pH 5.8: about 50% of maximal activity, pH 8.2: about 80% of maximal activity Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp.
-
-
 5.8