BRENDA - Enzyme Database show
show all sequences of 6.2.1.32

Purification and characterization of benzoate-coenzyme A ligase and 2-aminobenzoate-coenzyme A ligase from a denitrifying Pseudomonas sp.

Altenschmidt, U.; Oswald, B.; Fuchs, G.; J. Bacteriol. 173, 5494-5501 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
E2, no effect on isoenzyme E3
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
n-octylglucoside
-
Pseudomonas sp.
PCMB
isoenzyme E3, no effect on E2
Pseudomonas sp.
SDS
-
Pseudomonas sp.
Tween 100
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
2-Aminobenzoate
isoenzyme E2
Pseudomonas sp.
0.015
-
2-Aminobenzoate
isoenzyme E3
Pseudomonas sp.
0.02
-
CoA
isoenzyme E2
Pseudomonas sp.
0.026
-
CoA
isoenzyme E3
Pseudomonas sp.
0.035
-
Benzoate
isoenzyme E2
Pseudomonas sp.
0.04
-
ATP
isoenzyme E3
Pseudomonas sp.
0.05
-
2-Fluorobenzoate
isoenzyme E2
Pseudomonas sp.
0.07
-
2-Fluorobenzoate
isoenzyme E3
Pseudomonas sp.
0.075
-
Benzoate
isoenzyme E3
Pseudomonas sp.
0.083
-
ATP
isoenzyme E2
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas sp.
Mn2+
required, can replace Mg2+
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
isoenzyme E2, gel filtration
Pseudomonas sp.
65000
-
isoenzyme E3, gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + anthranilate + CoA
Pseudomonas sp.
isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism
AMP + diphosphate + anthranilyl-CoA
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB740
-
-
-
Oxidation Stability
Oxidation Stability
Organism
purified enzymes not sensitive to oxygen
Pseudomonas sp.
Purification (Commentary)
Commentary
Organism
strain KB740, 2 forms, E2: MW 60000, induced anaerobically by 2-aminobenzoate and E3: MW 65000, induced aerobically by 2-aminobenzoate
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:2-aminobenzoate-grown cell
anaerob and nitrate
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
4°C, 0.05 mg/ml protein concentration, Tris-HCl buffer, pH 7.8, 50% loss of activity after 4 days
Pseudomonas sp.
frozen in liquid nitrogen for 3 months without remarkable loss of activity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-fluorobenzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 2-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + 2-methylbenzoate + CoA
at less than 1/5 of the rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 2-methylbenzoyl-CoA + diphosphate
-
-
-
?
ATP + 3-fluorobenzoate + CoA
at half the reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 3-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + 4-fluorobenzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 4-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + anthranilate + CoA
isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism
713
Pseudomonas sp.
AMP + diphosphate + anthranilyl-CoA
-
713
Pseudomonas sp.
?
ATP + benzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + benzoyl-CoA + diphosphate
-
-
-
?
ATP + CoA + anthranilate
i.e. 2-aminobenzoate
713
Pseudomonas sp.
AMP + diphosphate + anthranilyl-CoA
-
713
Pseudomonas sp.
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, isoenzyme E2, 1 * 65000, isoenzyme E3, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
21.7
-
2-Aminobenzoate
28°C, isoenzyme E3
Pseudomonas sp.
267
-
2-Aminobenzoate
28°C, isoenzyme E2
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Pseudomonas sp.
8.6
-
isoenzyme E3
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
E2, no effect on isoenzyme E3
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
n-octylglucoside
-
Pseudomonas sp.
PCMB
isoenzyme E3, no effect on E2
Pseudomonas sp.
SDS
-
Pseudomonas sp.
Tween 100
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
2-Aminobenzoate
isoenzyme E2
Pseudomonas sp.
0.015
-
2-Aminobenzoate
isoenzyme E3
Pseudomonas sp.
0.02
-
CoA
isoenzyme E2
Pseudomonas sp.
0.026
-
CoA
isoenzyme E3
Pseudomonas sp.
0.035
-
Benzoate
isoenzyme E2
Pseudomonas sp.
0.04
-
ATP
isoenzyme E3
Pseudomonas sp.
0.05
-
2-Fluorobenzoate
isoenzyme E2
Pseudomonas sp.
0.07
-
2-Fluorobenzoate
isoenzyme E3
Pseudomonas sp.
0.075
-
Benzoate
isoenzyme E3
Pseudomonas sp.
0.083
-
ATP
isoenzyme E2
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas sp.
Mn2+
required, can replace Mg2+
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
isoenzyme E2, gel filtration
Pseudomonas sp.
65000
-
isoenzyme E3, gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + anthranilate + CoA
Pseudomonas sp.
isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism
AMP + diphosphate + anthranilyl-CoA
-
Pseudomonas sp.
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
purified enzymes not sensitive to oxygen
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
strain KB740, 2 forms, E2: MW 60000, induced anaerobically by 2-aminobenzoate and E3: MW 65000, induced aerobically by 2-aminobenzoate
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:2-aminobenzoate-grown cell
anaerob and nitrate
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.05 mg/ml protein concentration, Tris-HCl buffer, pH 7.8, 50% loss of activity after 4 days
Pseudomonas sp.
frozen in liquid nitrogen for 3 months without remarkable loss of activity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-fluorobenzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 2-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + 2-methylbenzoate + CoA
at less than 1/5 of the rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 2-methylbenzoyl-CoA + diphosphate
-
-
-
?
ATP + 3-fluorobenzoate + CoA
at half the reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 3-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + 4-fluorobenzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + 4-fluorobenzoyl-CoA + diphosphate
-
-
-
?
ATP + anthranilate + CoA
isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism
713
Pseudomonas sp.
AMP + diphosphate + anthranilyl-CoA
-
713
Pseudomonas sp.
?
ATP + benzoate + CoA
at the same reaction rate as 2-aminobenzoate
713
Pseudomonas sp.
AMP + benzoyl-CoA + diphosphate
-
-
-
?
ATP + CoA + anthranilate
i.e. 2-aminobenzoate
713
Pseudomonas sp.
AMP + diphosphate + anthranilyl-CoA
-
713
Pseudomonas sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, isoenzyme E2, 1 * 65000, isoenzyme E3, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
21.7
-
2-Aminobenzoate
28°C, isoenzyme E3
Pseudomonas sp.
267
-
2-Aminobenzoate
28°C, isoenzyme E2
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Pseudomonas sp.
8.6
-
isoenzyme E3
Pseudomonas sp.
Other publictions for EC 6.2.1.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744708
Witzgall
Structures of the N-terminal ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
ChemBioChem
18
2045-2055
2017
-
1
-
1
-
-
-
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1
-
2
-
5
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
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-
-
-
-
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1
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2
-
-
-
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-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
692840
Coleman
Pseudomonas aeruginosa PqsA is ...
Pseudomonas aeruginosa
J. Bacteriol.
190
1247-1255
2008
-
-
1
-
-
-
7
-
-
1
-
1
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
1
-
1
6
-
-
-
-
1
1
-
-
-
-
7
6
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
438855
Schuhle
Two similar gene clusters codi ...
Azoarcus evansii, Azoarcus evansii KB740
J. Bacteriol.
183
5268-5278
2001
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438858
Lochmeyer
Anaerobic degradation of 2-ami ...
Pseudomonas sp., Pseudomonas sp. KB740
J. Bacteriol.
174
3621-3628
1992
-
-
-
-
-
-
-
1
-
-
-
3
-
13
-
-
-
-
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713
Altenschmidt
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. KB740
J. Bacteriol.
173
5494-5501
1991
-
-
-
-
-
-
7
10
-
2
2
1
-
12
1
-
1
-
-
1
1
2
7
1
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
10
-
2
2
1
-
1
-
1
-
1
1
2
7
1
1
-
-
2
2
-
-
-
-
-
-
-
-
-
438854
Altenschmidt
Evidence that enzymes of a nov ...
Pseudomonas sp., Pseudomonas sp. KB740
Eur. J. Biochem.
194
647-653
1990
-
-
-
-
-
-
-
-
-
-
-
2
-
13
-
-
-
-
-
1
-
-
4
-
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2
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1
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4
-
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