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Literature summary for 6.2.1.32 extracted from

  • Altenschmidt, U.; Oswald, B.; Fuchs, G.
    Purification and characterization of benzoate-coenzyme A ligase and 2-aminobenzoate-coenzyme A ligase from a denitrifying Pseudomonas sp. (1991), J. Bacteriol., 173, 5494-5501.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.32

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) E2, no effect on isoenzyme E3 Pseudomonas sp.
Cu2+
-
 Pseudomonas sp.
n-octylglucoside
-
 Pseudomonas sp.
PCMB isoenzyme E3, no effect on E2 Pseudomonas sp.
SDS
-
 Pseudomonas sp.
Tween 100
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 2-Aminobenzoate isoenzyme E2 Pseudomonas sp.
0.015
-
 2-Aminobenzoate isoenzyme E3 Pseudomonas sp.
0.02
-
 CoA isoenzyme E2 Pseudomonas sp.
0.026
-
 CoA isoenzyme E3 Pseudomonas sp.
0.035
-
 benzoate isoenzyme E2 Pseudomonas sp.
0.04
-
 ATP isoenzyme E3 Pseudomonas sp.
0.05
-
 2-Fluorobenzoate isoenzyme E2 Pseudomonas sp.
0.07
-
 2-Fluorobenzoate isoenzyme E3 Pseudomonas sp.
0.075
-
 benzoate isoenzyme E3 Pseudomonas sp.
0.083
-
 ATP isoenzyme E2 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas sp.
Mn2+ required, can replace Mg2+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 isoenzyme E2, gel filtration Pseudomonas sp.
65000
-
 isoenzyme E3, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + anthranilate + CoA Pseudomonas sp. isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism AMP + diphosphate + anthranilyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB740
-
-
-

Oxidation Stability

Oxidation Stability Organism
purified enzymes not sensitive to oxygen Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
strain KB740, 2 forms, E2: MW 60000, induced anaerobically by 2-aminobenzoate and E3: MW 65000, induced aerobically by 2-aminobenzoate Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2-aminobenzoate-grown cell anaerob and nitrate Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
4°C, 0.05 mg/ml protein concentration, Tris-HCl buffer, pH 7.8, 50% loss of activity after 4 days Pseudomonas sp.
frozen in liquid nitrogen for 3 months without remarkable loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-fluorobenzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 2-fluorobenzoyl-CoA + diphosphate
-
 ?
ATP + 2-methylbenzoate + CoA at less than 1/5 of the rate as 2-aminobenzoate Pseudomonas sp. AMP + 2-methylbenzoyl-CoA + diphosphate
-
 ?
ATP + 3-fluorobenzoate + CoA at half the reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 3-fluorobenzoyl-CoA + diphosphate
-
 ?
ATP + 4-fluorobenzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + 4-fluorobenzoyl-CoA + diphosphate
-
 ?
ATP + anthranilate + CoA isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism Pseudomonas sp. AMP + diphosphate + anthranilyl-CoA
-
 ?
ATP + benzoate + CoA at the same reaction rate as 2-aminobenzoate Pseudomonas sp. AMP + benzoyl-CoA + diphosphate
-
 ?
ATP + CoA + anthranilate i.e. 2-aminobenzoate Pseudomonas sp. AMP + diphosphate + anthranilyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, isoenzyme E2, 1 * 65000, isoenzyme E3, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.7
-
 2-Aminobenzoate 28°C, isoenzyme E3 Pseudomonas sp.
267
-
 2-Aminobenzoate 28°C, isoenzyme E2 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Pseudomonas sp.
8.6
-
 isoenzyme E3 Pseudomonas sp.