Literature summary for 6.2.1.32 extracted from

Coleman, J.P.; Hudson, L.L.; McKnight, S.L.; Farrow, J.M.; Calfee, M.W.; Lindsey, C.A.; Pesci, E.C.
Pseudomonas aeruginosa PqsA is an anthranilate-coenzyme A ligase (2008), J. Bacteriol., 190, 1247-1255.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene pqsA is part of the pqsABCDE operon, sequence comparisons, expression of the His-tagged enzyme in Escherichia coli	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
2-amino-3-chlorobenzoate	-	Pseudomonas aeruginosa
3-fluoro-O-anisidine	-	Pseudomonas aeruginosa
5-nitroanthranilonitrile	-	Pseudomonas aeruginosa
anthranilonitrile	-	Pseudomonas aeruginosa
methylanthranilate	i.e. 2-aminobenzoate methyl ester	Pseudomonas aeruginosa
additional information	effects of substrate analogues on in vitro anthranilate-CoA ligase activity and in vivo PQS production in Pseudomonas aeruginosa, overview	Pseudomonas aeruginosa
salicylate	-	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + anthranilate + CoA	Pseudomonas aeruginosa	the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview	AMP + diphosphate + anthranilyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I4X3	gene pqsA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged PqsA 58fold from Escherichia coli by ammonium sulfate fractionation, metal affinity and hydrophobic interaction chromatography, and anion exchange chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + anthranilate + CoA	-	Pseudomonas aeruginosa	AMP + diphosphate + anthranilyl-CoA	-	?
ATP + anthranilate + CoA	the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview	Pseudomonas aeruginosa	AMP + diphosphate + anthranilyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
anthranilate-coenzyme A ligase	-	Pseudomonas aeruginosa
PqsA	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Pseudomonas aeruginosa

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0129	-	2-amino-3-chlorobenzoate	pH 8.0, 37°C	Pseudomonas aeruginosa
0.0183	-	salicylate	pH 8.0, 37°C	Pseudomonas aeruginosa
0.037	-	5-nitroanthranilonitrile	pH 8.0, 37°C	Pseudomonas aeruginosa
0.0897	-	3-fluoro-O-anisidine	pH 8.0, 37°C	Pseudomonas aeruginosa
1.3	-	anthranilonitrile	pH 8.0, 37°C	Pseudomonas aeruginosa
5.8	-	methylanthranilate	pH 8.0, 37°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)