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Literature summary for 6.2.1.30 extracted from
- Biosynthesis of Penicillin in vitro: purification & properties of phenyl/phenoxyacetic acid activating enzyme (1982), Indian J. Biochem. Biophys., 19, 257-261.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 0.01 mM, 100% stimulation | Penicillium chrysogenum |  |
| dithiothreitol | 0.01 mM, 80% stimulation | Penicillium chrysogenum | |
| GSH | 0.01 mM, 60% stimulation | Penicillium chrysogenum | |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Penicillium chrysogenum |
| repeated freezing and thawing inactivates | Penicillium chrysogenum |
| sucrose stabilizes | Penicillium chrysogenum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 0.01 mM, complete inhibition | Penicillium chrysogenum | |
| NEM | 0.01 mM, complete inhibition | Penicillium chrysogenum | |
| p-chloromercuribenzoate | 0.01 mM, complete inhibition | Penicillium chrysogenum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0029 | - |
phenylacetate | pH 6.8, 37°C | Penicillium chrysogenum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 0.01 mM, required, cannot be substituted by Mn2+, Zn2+, Na+, K+ | Penicillium chrysogenum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium chrysogenum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium chrysogenum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.199 | - |
- |
Penicillium chrysogenum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 24 h, very little activity retained, sucrose and 2-mercaptoethanol stabilize | Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phenoxyacetic acid + CoA | - |
Penicillium chrysogenum | AMP + phenoxyacetyl-CoA + diphosphate | - |
? | |
| ATP + phenoxyacetic acid + hydroxylamine | - |
Penicillium chrysogenum | AMP + phenylacetoxyhydroxamate + diphosphate | - |
? | |
| ATP + phenylacetate + CoA | - |
Penicillium chrysogenum | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
| ATP + phenylacetic acid + hydroxylamine | - |
Penicillium chrysogenum | AMP + phenylacetylhydroxamate + diphosphate | - |
? | |
| additional information | only beta-substitued acetic acid can serve as substrate, not phenylpropionic acid | Penicillium chrysogenum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Penicillium chrysogenum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | 42 | 32°C: about 40% of maximal activity, 42°C: about 40% of maximal activity | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Penicillium chrysogenum |
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