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Literature summary for 6.2.1.30 extracted from

  • El-Said Mohamed, M.; Fuchs, G.
    Purification and characterization of phenylacetyl-coenzyme A ligase from a denitrifying Pseudomonas sp., an enzyme involved in the anaerobic degradation of phenylacetate (1993), Arch. Microbiol., 159, 544-562 (c).
    View publication on PubMed
Search for more literature for 6.2.1.30

General Stability

General Stability Organism
enzyme is rather labile and requires the presence of glycerol and/or phenylacetate for stabilization Pseudomonas sp.
purified enzyme is sensitive to repeated freezing Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, 87% inhibition Pseudomonas sp.
iodoacetamide
-
 Pseudomonas sp.
Mo5+ 2 mM, 80% inhibition Pseudomonas sp.
NaF
-
 Pseudomonas sp.
NEM
-
 Pseudomonas sp.
Ni2+ 1 mM, 65% inhibition Pseudomonas sp.
p-hydroxymercuribenzoate
-
 Pseudomonas sp.
Zn2+ 2 mM, 84% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 phenylacetate pH 7.8, 30°C Pseudomonas sp.
0.15
-
 CoA pH 7.8, 30°C Pseudomonas sp.
0.29
-
 ATP pH 7.8, 30°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas sp.
Mg2+ optimal concentration: 5 mM Pseudomonas sp.
Mn2+ at 5 mM, 41% of the activation with Mg2+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Pseudomonas sp.
52000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Pseudomonas sp. first step in anaerobic degradation pathway of phenylacetate ?
-
 ?
ATP + phenylacetate + CoA Pseudomonas sp. KB 740 first step in anaerobic degradation pathway of phenylacetate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB 740
-
-
-

Oxidation Stability

Oxidation Stability Organism
insensitive to oxygen Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
strain KB 740 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture maximal activity in mid-exponential growth pase Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.4
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C or -70°C, 70% loss of activity after 1 week Pseudomonas sp.
4°C, 90% loss of activity after 1 day without glycerol, plus 10% glycerol, half-life: 2 days, plus 5 mM phenylacetate, half-life: 1 week, plus 35% glycerol, half-life: 2 weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phenylacetate + CoA highly specific Pseudomonas sp. AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA highly specific Pseudomonas sp. KB 740 AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA first step in anaerobic degradation pathway of phenylacetate Pseudomonas sp. ?
-
 ?
ATP + phenylacetate + CoA first step in anaerobic degradation pathway of phenylacetate Pseudomonas sp. KB 740 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.7
-
 ATP pH 7.8, 30°C Pseudomonas sp.
21.7
-
 CoA pH 7.8, 30°C Pseudomonas sp.
21.7
-
 phenylacetate pH 7.8, 30°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9 pH 8.5: activity maximum, pH 9.0: 50% of maximal activity Pseudomonas sp.