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Literature summary for 6.2.1.30 extracted from

  • Martinez-Blanco, H.; Reglero, A.; Rodriguez-Aparicio, L.B.; Luengo, J.M.
    Purification and biochemical characterization of phenylacetyl-CoA ligase from Pseudomonas putida (1990), J. Biol. Chem., 265, 7084-7090.
    View publication on PubMed
Search for more literature for 6.2.1.30

General Stability

General Stability Organism
purified enzyme is sensitive to repeated freezing Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 1 mM, 92% inhibition Pseudomonas putida
Cu2+ 1 mM, 97% inhibition Pseudomonas putida
Decanoic acid
-
 Pseudomonas putida
Hg2+ 1 mM, 95% inhibition Pseudomonas putida
n-octylglucoside
-
 Pseudomonas putida
NEM 1 mM, 99% inhibition Pseudomonas putida
Nonanoic acid
-
 Pseudomonas putida
Nonidet P-40
-
 Pseudomonas putida
octanoic acid
-
 Pseudomonas putida
p-chloromercuribenzoate 1 mM, 96% inhibition Pseudomonas putida
SDS
-
 Pseudomonas putida
Tween 100
-
 Pseudomonas putida
Zn2+ 1 mM, 95% inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 CoA pH 8.2, 30°C Pseudomonas putida
9.7
-
 ATP pH 8.2, 30°C Pseudomonas putida
16.5
-
 phenylacetate pH 8.2, 30°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas putida
Mn2+ 50% of the activation with Mg2+ Pseudomonas putida
additional information enzyme does not contain metal ions Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Pseudomonas putida enzyme is involved in the catabolism of phenylacetic acid ?
-
 ?
ATP + phenylacetate + CoA Pseudomonas putida specifically induced by phenylacetic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida
21
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, crude enzyme is stable in presence of glycerol for 2-3 weeks, more than 50% loss of activity of the purified enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phenylacetate + CoA 28% of the activity with ATP Pseudomonas putida ?
-
 ?
ATP + acetate + CoA 85% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + acetyl-CoA
-
 ?
ATP + butanoate + CoA 19% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + butanoyl-CoA
-
 ?
ATP + phenylacetate + CoA
-
 Pseudomonas putida AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA enzyme is involved in the catabolism of phenylacetic acid Pseudomonas putida ?
-
 ?
ATP + phenylacetate + CoA specifically induced by phenylacetic acid Pseudomonas putida ?
-
 ?
ATP + propanoate + CoA 47% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + propanoyl-CoA
-
 ?
CTP + phenylacetate + CoA 2.8% of the activity with ATP Pseudomonas putida CMP + diphosphate + phenylacetyl-CoA
-
 ?
additional information no activity with: 3-hydroxyphenylacetic acid, 4-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid Pseudomonas putida ?
-
 ?
UTP + phenylacetate + CoA 2.5% of the activity with ATP Pseudomonas putida UMP + diphosphate + phenylacetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 glycerol, 20% v/v, ATP, MgCl2 or phenylacetic acid protects against thermal inactivation Pseudomonas putida
20
-
 45 min stable, about 15% loss of activity after 60 min Pseudomonas putida
25
-
 30 min, about 20% loss of activity Pseudomonas putida
30
-
 60 min, about 40% loss of activity Pseudomonas putida
37
-
 60 min, about 75% loss of activity Pseudomonas putida
45
-
 15 min, about 90% loss of activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Pseudomonas putida

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida isoelectric focusing
-
 5.4