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Literature summary for 6.2.1.3 extracted from
- Purification and properties of long-chain acyl-coenzyme -A synthetase from rat liver (1979), Eur. J. Biochem., 98, 165-172.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fatty acids | palmitoleate, oleate and linoleate are competitive inhibitors of the activation of each other | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | 0.028 | CoA | - |
Rattus norvegicus | |
| 0.23 | 0.29 | dephospho-CoA | - |
Rattus norvegicus | |
| 0.41 | 0.43 | 4'-phosphopantetheine | - |
Rattus norvegicus | |
| 1.1 | 1.2 | pantetheine | - |
Rattus norvegicus | |
| 2.3 | 2.4 | ATP | - |
Rattus norvegicus | |
| 12.5 | 13.3 | dATP | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.2 | - |
mitochondrial enzyme | Rattus norvegicus |
| 28.7 | - |
microsomal enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 4 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + fatty acid + 4'-phosphopantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-4'-phosphopantetheine | - |
? | |
| ATP + fatty acid + dephospho-CoA | - |
Rattus norvegicus | AMP + diphosphate + acyl-dephospho-CoA | - |
? | |
| ATP + fatty acid + pantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-pantetheine | - |
? | |
| ATP + long-chain carboxylic acid + CoA | substrate specificity of microsomal and mitochondrial enzyme are indistinguishable | Rattus norvegicus | AMP + diphosphate + long-chain acyl-CoA | - |
? | |
| ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? | |
| dATP + fatty acid + CoA | - |
Rattus norvegicus | dAMP + diphosphate + acyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
2.5 min: 17-20% loss of activity, 5 min: 33-37% loss of activity, 15 min: 58-59% loss of activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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