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Literature summary for 6.2.1.3 extracted from
- Long-chain acyl-CoA synthetase from rat liver (1981), Methods Enzymol., 71, 334-341.
General Stability
| General Stability | Organism |
|---|---|
| stable to 24 h dialysis at 0EC, against 50 mM potassium phosphate buffer, pH 7.4, containing 2 mM Triton X-100, 2 mM DTT, 1 mM EDTA | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | 0.028 | CoA | - |
Rattus norvegicus |  |
| 0.23 | 0.29 | dephospho-CoA | - |
Rattus norvegicus | |
| 0.41 | 0.43 | 4'-phosphopantetheine | - |
Rattus norvegicus | |
| 1.1 | 1.2 | pantetheine | - |
Rattus norvegicus | |
| 2.3 | 2.4 | ATP | - |
Rattus norvegicus | |
| 12.5 | 13.3 | dATP | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.2 | - |
mitochondrial enzyme | Rattus norvegicus |
| 28.7 | - |
microsomal enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 4 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + fatty acid + 4'-phosphopantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-4'-phosphopantetheine | - |
? | |
| ATP + fatty acid + dephospho-CoA | - |
Rattus norvegicus | AMP + diphosphate + acyl-dephospho-CoA | - |
? | |
| ATP + fatty acid + pantetheine | - |
Rattus norvegicus | AMP + diphosphate + acyl-pantetheine | - |
? | |
| dATP + fatty acid + CoA | - |
Rattus norvegicus | dAMP + diphosphate + acyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.1 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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