KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
stearate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.0002 | - |
myristate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.00021 | - |
palmitate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.00028 | - |
stearate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.00051 | - |
laurate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.00072 | - |
Octanoate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.0012 | - |
myristate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.0019 | - |
laurate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.002 | - |
laurate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.0021 | - |
myristate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.0029 | - |
palmitate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.004 | - |
Decanoate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.0059 | - |
Octanoate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.0063 | - |
Decanoate | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.0083 | - |
Decanoate | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.0091 | - |
stearate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.0125 | - |
Octanoate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.0143 | - |
palmitate | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.025 | - |
ATP | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.033 | - |
ATP | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.034 | - |
CoA | acyl-CoA synthetase-5.6 | Escherichia coli | |
0.035 | - |
CoA | acyl-CoA synthetase-5.0 | Escherichia coli | |
0.05 | - |
ATP | acyl-CoA synthetase-4.6 | Escherichia coli | |
0.053 | - |
CoA | acyl-CoA synthetase-4.6 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
multiple charge isoforms: 4.6, 5.0 and 5.6 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | enzyme does not contain carbohydrate, phospholipid and fatty acid | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + decanoate + CoA | - |
Escherichia coli | AMP + diphosphate + decanoyl-CoA | - |
? | |
ATP + laurate + CoA | - |
Escherichia coli | AMP + diphosphate + lauroyl-CoA | - |
? | |
ATP + myristate + CoA | - |
Escherichia coli | AMP + diphosphate + myristoyl-CoA | - |
? | |
ATP + octanoate + CoA | - |
Escherichia coli | AMP + diphosphate + octanoyl-CoA | - |
? | |
ATP + palmitate + CoA | - |
Escherichia coli | AMP + diphosphate + palmitoyl-CoA | - |
? | |
ATP + stearate + CoA | - |
Escherichia coli | AMP + diphosphate + stearoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
5 min: 22% loss of acyl-CoA synthetase-4.6 activity, 38% loss of acyl-CoA synthetase-5.6 activity, 80% loss of acyl-CoA synthetase 5.0 activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.9 | acyl-CoA synthetase-5.0 | Escherichia coli |
8.1 | - |
acyl-CoA synthetase-4.6 and acyl-CoA synthetase-5.6. Acyl-CoA synthetase-4.6 has a second optimum at pH 8.5-9.4 | Escherichia coli |
8.5 | 9.4 | acyl-CoA synthetase-4.6 has a second optimum at pH 8.1 | Escherichia coli |