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Literature summary for 6.2.1.3 extracted from

  • Soupene, E.; Dinh, N.P.; Siliakus, M.; Kuypers, F.A.
    Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains (2010), BMC Biochem., 11, 18.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information specific Gate-domain residues are essential for activity, detailed overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
ACSL6 isoforms and truncated constructs, splicing variants, sequence organization, overview, expression of His-tagged full-length ACSL6 isoform 2 (F-Gate) and DELTAN-truncated version of isoform 1 (DELTAN-(Y-Gate)) in Escherichia coli strain BL21(DE3) membranes Homo sapiens

Protein Variants

Protein Variants Comment Organism
F319A site-directed mutagenesis Homo sapiens
F319W site-directed mutagenesis Homo sapiens
F319Y site-directed mutagenesis Homo sapiens
H316L site-directed mutagenesis Homo sapiens
L316H site-directed mutagenesis Homo sapiens
additional information mutants of full-length ACSL6 isoform 2 (F-Gate) and DELTAN-truncated version of isoform 1 (DELTAN-(Y-Gate)) are obtained by site-directed mutagenesis of the H/L residue pair at position 316 and of the F/Y residue pair at position 319. An ACSL6 construct with a tag at its C-terminus has a far lower activity than a construct with a tag fused to its N-terminus Homo sapiens
Y319A site-directed mutagenesis Homo sapiens
Y319F site-directed mutagenesis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information an ACSL6 construct with a tag at its C-terminus has a far lower activity than a construct with a tag fused to its N-terminus Homo sapiens
Triton X-100 Inhibition of acyl-CoA formation, inhibits ACSL6 isoform 2 (F-Gate) and its N-terminus truncated version, DELTAN-(F-Gate) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane isozyme ACSL6 Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acylate + CoA Homo sapiens
-
AMP + diphosphate + acyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme ACSL6
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acylate + CoA
-
Homo sapiens AMP + diphosphate + acyl-CoA
-
?

Synonyms

Synonyms Comment Organism
ACSL6
-
Homo sapiens
Acyl-CoA synthetase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
additional information long-chain acyl-CoA synthetase member 6, ASCL6, is a form present in the plasma membrane of cells. Splicing events affecting the N-terminus and alternative motifs near the ATP-binding site generate different isoforms of ACSL6. Alternative fatty acid Gate-domain motifs are essential determinants for the activity of the human ACSL6 isoforms, which appear to act as homodimeric enzyme as well as in complex with other spliced forms. The diversity of these enzyme species can produce the variety of acyl-CoA synthetase activities that are necessary to generate and repair the hundreds of lipid species present in membranes. Oligomeric complex fomations and interactions between isoforms, overview. The N-terminal domain is not essential for oligomer formation Homo sapiens