Cloned (Comment) | Organism |
---|---|
gene acsA, expression of His-tagged enzyme | Pseudomonas chlororaphis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas chlororaphis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | Q5CD72 | gene acsA | - |
Pseudomonas chlororaphis B23 | Q5CD72 | gene acsA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AcsA by nickel affinity chromatography | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also forms a carbon-nitrogen bond, reaction of EC 6.3.1 acid-ammonia (or amide) ligase, i.e. amide synthase, and EC 6.3.2 acid-amino acid ligase, i.e. peptide synthase, comprising the amino group of the cysteine and the carboxyl group of the acid, overview | Pseudomonas chlororaphis | ? | - |
? | |
additional information | the enzyme also forms a carbon-nitrogen bond, reaction of EC 6.3.1 acid-ammonia (or amide) ligase, i.e. amide synthase, and EC 6.3.2 acid-amino acid ligase, i.e. peptide synthase, comprising the amino group of the cysteine and the carboxyl group of the acid, overview | Pseudomonas chlororaphis B23 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcsA | - |
Pseudomonas chlororaphis |
Acyl-CoA synthetase | - |
Pseudomonas chlororaphis |
More | acyl-CoA synthetase belongs to the superfamily of adenylate-forming enzymes, whose three-dimensional structures are analogous to one another | Pseudomonas chlororaphis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas chlororaphis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas chlororaphis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas chlororaphis |