Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0183 | - |
CoA | in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C | Saccharomyces cerevisiae | |
0.0516 | - |
ATP | in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C | Saccharomyces cerevisiae | |
0.0711 | - |
oleate | in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 10 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72500 | - |
2 * 72500, gel filtration | Saccharomyces cerevisiae |
145000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Sephacryl S-200 HR gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.121 | - |
in the presence of 10 mM MnCl2, at pH 8.0 | Saccharomyces cerevisiae |
0.145 | - |
in the presence of 10 mM MgCl2, at pH 8.0 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oleate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + oleoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 72500, gel filtration | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ACSL | - |
Saccharomyces cerevisiae |
Faa1p | - |
Saccharomyces cerevisiae |
fatty acid CoA ligase: AMP forming | - |
Saccharomyces cerevisiae |
long chain acyl-CoA synthetase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |