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Literature summary for 6.2.1.3 extracted from

  • Li, H.; Melton, E.M.; Quackenbush, S.; DiRusso, C.C.; Black, P.N.
    Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae (2007), Biochim. Biophys. Acta, 1771, 1246-1253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0183
-
CoA in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae
0.0516
-
ATP in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae
0.0711
-
oleate in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 10 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72500
-
2 * 72500, gel filtration Saccharomyces cerevisiae
145000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Sephacryl S-200 HR gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.121
-
in the presence of 10 mM MnCl2, at pH 8.0 Saccharomyces cerevisiae
0.145
-
in the presence of 10 mM MgCl2, at pH 8.0 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oleate + CoA
-
Saccharomyces cerevisiae AMP + diphosphate + oleoyl-CoA
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 72500, gel filtration Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ACSL
-
Saccharomyces cerevisiae
Faa1p
-
Saccharomyces cerevisiae
fatty acid CoA ligase: AMP forming
-
Saccharomyces cerevisiae
long chain acyl-CoA synthetase
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae