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Literature summary for 6.2.1.3 extracted from

  • Van Horn, C.G.; Caviglia, J.M.; Li, L.O.; Wang, S.; Granger, D.A.; Coleman, R.A.
    Characterization of recombinant long-chain rat acyl-CoA synthetase isoforms 3 and 6: identification of a novel variant of isoform 6 (2005), Biochemistry, 44, 1635-1642.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzyme ACSL6_v1 in Escherichia coli Rattus norvegicus
expression of isoenzyme ACSL6_v2 in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information isoenzyme ACSL3 maintains activity in presence of 2-3 mM Triton X-100. Isoenzyme ACSL6_v1 is insensitive to rosiglitazone; isoenzyme ACSL6_v1 maintains activity in presence of 2-3 mM Triton X-100. Isoenzyme ACSL6_v1 is insensitive to rosiglitazone and is not affected by triacsin C up to concentrations of 0.05 mM; isoenzyme ACSL6_v2 maintains optimal activity up to 4 mM Triton X-100. Isoenzyme ACSL6_v2 is insensitive to rosiglitazone and is not affected by triacsin C up to concentrations of 0.05 mM Rattus norvegicus
Triacsin C IC50: 0.0055 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
CoA pH 7.4, isoenzyme ACSL6_v2 Rattus norvegicus
0.003
-
CoA pH 7.4, isoenzyme ACSL3 Rattus norvegicus
0.0036
-
palmitate pH 7.4, isoenzyme ACSL6_v2 Rattus norvegicus
0.0043
-
palmitate pH 7.4, isoenzyme ACSL3 Rattus norvegicus
0.0051
-
oleate pH 7.4, isoenzyme ACSL3 Rattus norvegicus
0.0053
-
oleate pH 7.4, isoenzyme ACSL6_v2 Rattus norvegicus
0.0065
-
arachidonate pH 7.4, isoenzyme ACSL6_v2 Rattus norvegicus
0.0089
-
arachidonate pH 7.4, isoenzyme ACSL3 Rattus norvegicus
0.402
-
ATP pH 7.4, isoenzyme ACSL3 Rattus norvegicus
1.48
-
ATP pH 7.4, isoenzyme ACSL6_v2 Rattus norvegicus
3
-
oleate pH 7.4, isoenzyme ACSL6_v1 Rattus norvegicus
4.7
-
CoA pH 7.4, isoenzyme ACSL6_v1 Rattus norvegicus
6
-
palmitate pH 7.4, isoenzyme ACSL6_v1 Rattus norvegicus
9.7
-
arachidonate pH 7.4, isoenzyme ACSL6_v1 Rattus norvegicus
12.21
-
ATP pH 7.4, isoenzyme ACSL6_v1 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P33124 isoenzyme ACSL3
-
Rattus norvegicus P33124 isoenzyme ACSL6_v1
-
Rattus norvegicus P33124 isoenzyme ACSL6_v2
-

Source Tissue

Source Tissue Comment Organism Textmining
brain ACSL3 and ACSL6 are the predominant isoforms in brain Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + arachidonate + CoA
-
Rattus norvegicus AMP + diphosphate + arachidonoyl-CoA
-
?
ATP + oleate + CoA
-
Rattus norvegicus AMP + diphosphate + oleoyl-CoA
-
?
ATP + palmitate + CoA
-
Rattus norvegicus AMP + diphosphate + palmitoyl-CoA
-
?

Synonyms

Synonyms Comment Organism
ACSL3
-
Rattus norvegicus
ACSL6_v1
-
Rattus norvegicus
ACSL6_v2
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
half-life of isoenzyme ACSL6_v1 is 5 min Rattus norvegicus
43
-
half-life of isoenzyme ACSL6_v2 is 5 min Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0055
-
IC50: 0.0055 mM Rattus norvegicus Triacsin C