BRENDA - Enzyme Database
show all sequences of 6.2.1.27

Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying bacterium Thauera aromatica

Laempe, D.; Jahn, M.; Breese, K.; Schägger, H.; Fuchs, G.; J. Bacteriol. 183, 968-979 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Thauera aromatica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
4-hydroxybenzoate
pH 7.8, 30°C
Thauera aromatica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 4-hydroxybenzoate + CoA
Thauera aromatica
the enzyme is involved in the anaerobic hydroxybenzoate metabolism
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thauera aromatica
Q9AJS8
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thauera aromatica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.12
-
pH 7.8, 30°C
Thauera aromatica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 4-hydroxybenzoate + CoA
the enzyme is involved in the anaerobic hydroxybenzoate metabolism
712273
Thauera aromatica
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
-
?
ATP + 4-hydroxybenzoate + CoA
the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate
712273
Thauera aromatica
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
-
?
additional information
the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates
712273
Thauera aromatica
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Thauera aromatica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates
Thauera aromatica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9
Thauera aromatica
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thauera aromatica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
4-hydroxybenzoate
pH 7.8, 30°C
Thauera aromatica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 4-hydroxybenzoate + CoA
Thauera aromatica
the enzyme is involved in the anaerobic hydroxybenzoate metabolism
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thauera aromatica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.12
-
pH 7.8, 30°C
Thauera aromatica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 4-hydroxybenzoate + CoA
the enzyme is involved in the anaerobic hydroxybenzoate metabolism
712273
Thauera aromatica
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
-
?
ATP + 4-hydroxybenzoate + CoA
the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate
712273
Thauera aromatica
AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
-
-
?
additional information
the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates
712273
Thauera aromatica
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Thauera aromatica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates
Thauera aromatica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9
Thauera aromatica
Expression
Organism
Commentary
Expression
Thauera aromatica
induction during anaerobic growth with by 3-hydroxybenzoate
up
Expression (protein specific)
Organism
Commentary
Expression
Thauera aromatica
induction during anaerobic growth with by 3-hydroxybenzoate
up
Other publictions for EC 6.2.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674335
Peters
Genes, enzymes, and regulation ...
Geobacter metallireducens
J. Bacteriol.
189
4729-4738
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712275
Ding
Anaerobic metabolism of catech ...
Azoarcus sp., Azoarcus sp. EbN1, Thauera aromatica
J. Bacteriol.
190
1620-1630
2007
-
-
2
-
-
-
-
1
-
-
2
3
-
3
-
-
2
-
-
1
1
-
6
2
2
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
2
3
-
-
-
2
-
1
1
-
6
2
-
-
-
1
-
-
-
-
-
-
-
-
1
1
712273
Laempe
Anaerobic metabolism of 3-hydr ...
Thauera aromatica
J. Bacteriol.
183
968-979
2001
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
1
1
-
-
1
-
-
1
-
-
288500
Heider
Differential induction of enzy ...
Thauera aromatica
Arch. Microbiol.
170
120-131
1998
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
595
Barillas
3-Hydroxybenzoate:coenzyme A l ...
Centaurium erythraea
Planta
202
112-116
1997
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
16327
Schmidt
Alternative pathways of xantho ...
Hypericum androsaemum
FEBS Lett.
420
143-146
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
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-
-
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-
-
-
-
-
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-
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-
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-
1
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-
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-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
648909
Biegert
Enzymes of anarobic metabolism ...
Pseudomonas sp., Pseudomonas sp. K172
Eur. J. Biochem.
213
555-561
1993
-
-
-
-
-
-
4
3
-
-
1
2
-
10
1
-
1
-
-
1
1
-
13
1
-
-
-
-
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
4
2
3
-
-
1
2
-
1
-
1
-
1
1
-
13
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
648907
Merkel
Involvement of coenzyme A thio ...
Rhodopseudomonas palustris
J. Bacteriol.
171
1-7
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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1
1
-
2
-
-
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-
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-
-
-
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-
-
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-
-