KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0165 | - |
CoA | pH 7.2, 30°C | Mycolicibacterium phlei | |
0.1481 | - |
o-succinylbenzoate | pH 7.2, 30°C | Mycolicibacterium phlei | |
0.4 | - |
ATP | mutant enzyme H341A | Mycolicibacterium phlei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 8% of the activation with Mg2+ | Mycolicibacterium phlei | |
Co2+ | 63% of the activation with Mg2+ | Mycolicibacterium phlei | |
Cu2+ | 7% of the activation with Mg2+ | Mycolicibacterium phlei | |
Mg2+ | activation | Mycolicibacterium phlei | |
Mn2+ | 36% of the activation with Mg2+ | Mycolicibacterium phlei | |
Ni2+ | 46% of the activation with Mg2+ | Mycolicibacterium phlei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Mycolicibacterium phlei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-succinylbenzoate + CoA | Mycolicibacterium phlei | o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium phlei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00084 | - |
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-succinylbenzoate + CoA | - |
Mycolicibacterium phlei | AMP + diphosphate + 2-succinylbenzoyl-CoA | incubation of the purified enzyme at different pH values gives a varying ratio of o-succinylbenzoyl-CoA to o-succinylbenzoyl-diCoA. No formation of o-succinylbenzoyl-2-CoA. No formation of ADP | ? | |
ATP + 2-succinylbenzoate + CoA | o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis | Mycolicibacterium phlei | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? | |
ATP + 2-succinylbenzoate + dephospho-CoA | 96% of the activity with CoA | Mycolicibacterium phlei | AMP + 2-succinylbenzoyldephospho-CoA + diphosphate | - |
? | |
ATP + benzoylpropionic acid + CoA | 21.6% of the activity with o-succinylbenzoate | Mycolicibacterium phlei | AMP + benzoylpropionyl-CoA + diphosphate | - |
? | |
ATP + o-malonylbenzoate + CoA | 11.6% of the activity with o-succinylnemzoate | Mycolicibacterium phlei | AMP + o-malonylbenzoyl-CoA + diphosphate | - |
? | |
CTP + o-succinylbenzoate + CoA | 13.5% of the activity with ATP | Mycolicibacterium phlei | CMP + diphosphate + o-succinylbenzoyl-CoA | - |
? | |
GTP + o-succinylbenzoate + CoA | 13.4% of the activity with ATP | Mycolicibacterium phlei | GMP + diphosphate + o-succinylbenzoyl-CoA | - |
? | |
ITP + o-succinylbenzoate + CoA | 10.9% of the activity with ATP | Mycolicibacterium phlei | IMP + diphosphate + o-succinylbenzoyl-CoA | - |
? | |
TTP + o-succinylbenzoate + CoA | 12.1% of the activity with ATP | Mycolicibacterium phlei | TMP + diphosphate + o-succinylbenzoyl-CoA | - |
? | |
UTP + o-succinylbenzoate + CoA | 12.3% of the activity with ATP | Mycolicibacterium phlei | UMP + diphosphate + o-succinylbenzoyl-CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Mycolicibacterium phlei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycolicibacterium phlei | isoelectric focusing | - |
4.9 |