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Literature summary for 6.2.1.25 extracted from

  • Gibson, J.; Geissler, J.F.; Harwood, C.S.
    Benzoate-CoA ligase from Rhodopseudomonas palustris (1990), Methods Enzymol., 188, 154-159.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
ATP
-
Rhodopseudomonas palustris
0.1
-
CoASH
-
Rhodopseudomonas palustris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodopseudomonas palustris
Mn2+ can replace Mg2+ Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 62000 HPLC gel filtration Rhodopseudomonas palustris

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
Rhodopseudomonas palustris

Storage Stability

Storage Stability Organism
in liquid N2, no greater losses of activity after 3-6 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobenzoate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + 2-aminobenzoyl-CoA
-
?
ATP + 2-fluorobenzoate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + 2-fluorobenzoyl-CoA
-
?
ATP + 4-fluorobenzoate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + 4-fluorobenzoyl-CoA
-
?
ATP + benzoate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + benzoyl-CoA
-
?
ATP + DELTA1-cyclohexenecarboxylate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
-
?
ATP + DELTA3-cyclohexenecarboxylate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
-
?
ATP + picolinate + CoA
-
Rhodopseudomonas palustris AMP + diphosphate + picolinyl-CoA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000-62000, denaturing electrophoresis Rhodopseudomonas palustris