KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
benzoate | apparent value, at pH 9.0 | Magnetospirillum sp. | |
0.092 | - |
CoA | apparent value, at pH 9.0 | Magnetospirillum sp. | |
0.265 | - |
ATP | apparent value, at pH 9.0 | Magnetospirillum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, gel filtration | Magnetospirillum sp. |
120000 | - |
SDS-PAGE | Magnetospirillum sp. |
126000 | - |
gel filtration | Magnetospirillum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Magnetospirillum sp. | Q25C16 | strainTS-6 | - |
Magnetospirillum sp. TS-6 | Q25C16 | strainTS-6 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, Phenyl-Sepharose Fast Flow column chromatography, and Superose 12 gel filtration | Magnetospirillum sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.081 | - |
soluble protein, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10mM KCl, 2.5mM MgCl2, 10mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2U of lactate dehydrogenase, and 0.1 mM substrate | Magnetospirillum sp. |
13.4 | - |
after 166fold purification, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10 mM KCl, 2.5 mM MgCl2, 10 mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2 mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2 units of lactate dehydrogenase, and 0.1 mM substrate | Magnetospirillum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-aminobenzoate + CoA | 6.7% and 10% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 2-aminobenzoyl-CoA | - |
? | |
ATP + 2-fluorobenzoate + CoA | 61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 2-fluorobenzoate + CoA | 61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. TS-6 | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 3-aminobenzoate + CoA | 3.7% activity in anaerobically grown cells, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 3-aminobenzoyl-CoA | - |
? | |
ATP + 3-fluorobenzoate + CoA | 16% activity in aerobically and anaerobically grown cells, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
ATP + 3-fluorobenzoate + CoA | 16% activity in aerobically and anaerobically grown cells, compared to the activity with benzoate | Magnetospirillum sp. TS-6 | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
ATP + 4-aminobenzoate + CoA | 5.6% activity in anaerobically grown cells, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 4-aminobenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. TS-6 | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + benzoate + CoA | 100% activity in aerobically and anaerobically grown cells | Magnetospirillum sp. | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + benzoate + CoA | 100% activity in aerobically and anaerobically grown cells | Magnetospirillum sp. TS-6 | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + picolinate + CoA | 3.4% and 2.7% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate | Magnetospirillum sp. | AMP + diphosphate + picolinyl-CoA | - |
? | |
additional information | little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate | Magnetospirillum sp. | ? | - |
? | |
additional information | little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate | Magnetospirillum sp. TS-6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 63000, gel filtration | Magnetospirillum sp. |
Synonyms | Comment | Organism |
---|---|---|
Bcl | - |
Magnetospirillum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Magnetospirillum sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Magnetospirillum sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Magnetospirillum sp. |