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Literature summary for 6.2.1.22 extracted from

  • Antranikian, G.; Gottschalk, G.
    Copurification of citrate lyase and citrate lyase ligase from Rhodopseudomonas gelatinosa and subsequent separation of the two enzymes (1982), Eur. J. Biochem., 126, 43-47.
    View publication on PubMed
Search for more literature for 6.2.1.22

General Stability

General Stability Organism
stabilized by nucleotides, most potent stabilizer is ADP Rubrivivax gelatinosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000037
-
 citrate lyase sulfhydryl form Rubrivivax gelatinosus
0.14
-
 ATP
-
 Rubrivivax gelatinosus
5
-
 acetate
-
 Rubrivivax gelatinosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration: 3 mM, inhibitory above 5 mM Rubrivivax gelatinosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
 gel filtration, density gradient centrifugation Rubrivivax gelatinosus
41690
-
 1 * 41690, SDS-PAGE Rubrivivax gelatinosus

Organism

Organism UniProt Comment Textmining
Rubrivivax gelatinosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurification of citrate lyase and citrate lyase ligase Rubrivivax gelatinosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rubrivivax gelatinosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase Rubrivivax gelatinosus AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
ATP + acetate + citrate (pro-3S)-lyase highly specific, cannot react with citrate lyases from nonphototrophic microorganisms, e.g. Enterobacter aerogenes, Clostridium sphenoides or Streptococcus diacetilactis Rubrivivax gelatinosus AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
ATP + acetate + citrate lyase
-
 Rubrivivax gelatinosus AMP + diphosphate + acetyl-[citrate lyase]
-
 ?
ATP + propionate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, propionate at 47% of the activity relative to acetate Rubrivivax gelatinosus AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
CTP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, CTP at 30% of the relative to ATP Rubrivivax gelatinosus CMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
dTTP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, dTTP at 5% of the activity relative to ATP Rubrivivax gelatinosus dTMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
GTP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, GTP at 43% of the activity of ATP Rubrivivax gelatinosus GMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
ITP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, ITP at 46% of the activity relative to ATP Rubrivivax gelatinosus IMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
UTP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, UTP at 18% of the activity relative to ATP Rubrivivax gelatinosus UMP + diphosphate + citrate(pro-3S)-lyase
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41690, SDS-PAGE Rubrivivax gelatinosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Rubrivivax gelatinosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 6: 50% of maximal activity, 8: 55% of maximal activity Rubrivivax gelatinosus