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Literature summary for 6.2.1.22 extracted from
- Covalent modification of citrate lyase ligase from Clostridium sphenoides by phosphorylation/dephosphorylation (1985), Eur. J. Biochem., 153, 413-420.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Lacrimispora sphenoides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
ATP | - |
Lacrimispora sphenoides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal Mg2+ concentration: 1.6-1.9 mM | Lacrimispora sphenoides | |
| PO43- | activity is modulated by phosphorylation/dephosphorylation, active form of enzyme is phosphorylated | Lacrimispora sphenoides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
1 * 56000, SDS-PAGE | Lacrimispora sphenoides |
| 58000 | - |
gel filtration, sucrose density gradient centrifugation | Lacrimispora sphenoides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + citrate (pro-3S)-lyase | Lacrimispora sphenoides | thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate | ? | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | Lacrimispora sphenoides C2 / DSM 614 | thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacrimispora sphenoides | - |
- |
- |
| Lacrimispora sphenoides C2 / DSM 614 | - |
- |
- |
| Lactococcus lactis | - |
- |
- |
| [Clostridium] sporosphaeroides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lacrimispora sphenoides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lacrimispora sphenoides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase | Lacrimispora sphenoides | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase | [Clostridium] sporosphaeroides | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase | Lactococcus lactis | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase | Lacrimispora sphenoides C2 / DSM 614 | AMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate | Lacrimispora sphenoides | ? | - |
? | |
| ATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate | Lacrimispora sphenoides C2 / DSM 614 | ? | - |
? | |
| dATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, dATP at 72% of the activity relative to ATP | Lacrimispora sphenoides | dAMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| dATP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, dATP at 72% of the activity relative to ATP | Lacrimispora sphenoides C2 / DSM 614 | dAMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ITP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, ITP at 33% of the activity relative to ATP | Lacrimispora sphenoides | IMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
| ITP + acetate + citrate (pro-3S)-lyase | thiol form of (pro-3S)-lyase, ITP at 33% of the activity relative to ATP | Lacrimispora sphenoides C2 / DSM 614 | IMP + diphosphate + citrate(pro-3S)-lyase | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56000, SDS-PAGE | Lacrimispora sphenoides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Lacrimispora sphenoides |
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