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Literature summary for 6.2.1.20 extracted from
- Solubilization, purification, and salt activation of acyl-acyl carrier protein synthetase from Escherichia coli (1979), J. Biol. Chem., 254, 7116-7122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no evidence for a phospholipid requirement | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| ATP protects against heat inactivation at 55°C | Escherichia coli |
| Mg2+ is not required for ATP to protect the enzyme from heat denaturation | Escherichia coli |
| salts destabilize the enzyme, which results in increased heat lability | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
acyl-carrier protein | - |
Escherichia coli |  |
| 0.0053 | - |
palmitic acid | - |
Escherichia coli | |
| 0.03 | - |
oleic acid | - |
Escherichia coli | |
| 0.3 | - |
ATP | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inner membrane | - |
Escherichia coli | - |
- |
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Salt | requirement for high concentrations of salt, with the exception of Mg2+, the requirement for ion is nonspecific. Chaotrophic salts are effective at lower concentrations than less chaotrophic salts | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + oleic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + oleoyl-[acyl-carrier protein] | - |
? | |
| ATP + palmitic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + palmitoyl-[acyl-carrier protein] | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life: 2 min, in presence of ATP the half-life increases to above 32 min | Escherichia coli |
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