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Literature summary for 6.2.1.20 extracted from

  • Shanklin, J.
    Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form (2000), Protein Expr. Purif., 18, 355-360.
    View publication on PubMed
Search for more literature for 6.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and C41(DE3) Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane inner membrane Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80600
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + an acid + [acyl-carrier protein] Escherichia coli synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates, donor for activated acyl groups for the synthesis of lipids and lipid derivatives, functions as 2-acylglycerophosphoethanolamine acyltransferase AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
 transfected Escherichia coli BL21(DE3) with lactose as inducer and palmitic acid as substrate Escherichia coli
0.071
-
 transfected Escherichia coli C41(DE3) with IPTG as inducer and palmitic acid as substrate Escherichia coli
1.32
-
 purified enzyme from Escherichia coli C41(DE3) with palmitic acid as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + an acid + [acyl-carrier protein] synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates, donor for activated acyl groups for the synthesis of lipids and lipid derivatives, functions as 2-acylglycerophosphoethanolamine acyltransferase Escherichia coli AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
ATP + an acid + [acyl-carrier protein] fatty acids with chain lengths between 8 and 18 carbons serve as substrates Escherichia coli AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?