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Literature summary for 6.2.1.17 extracted from

  • Alber, B.E.; Kung, J.W.; Fuchs, G.
    3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation (2008), J. Bacteriol., 190, 1383-1389.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.17

Activating Compound

Activating Compound Comment Organism Structure
additional information 3-hydroxypropionyl-CoA synthetase is up-regulated eightfold in autotrophically versus heterotrophically grown Metallosphaera sedula Metallosphaera sedula

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the dependency of the 3-hydroxypropionyl-CoA synthetase with respect to CoA does not follow Michaelis-Menten kinetics, overview Metallosphaera sedula
0.045
-
 ATP pH 8.4, 65°C Metallosphaera sedula
0.11
-
 ATP pH 8.4, 65°C Sulfurisphaera tokodaii
0.12
-
 propanoate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
0.18
-
 3-hydroxypropanoate pH 8.4, 65°C Metallosphaera sedula
0.19
-
 3-hydroxypropanoate pH 8.4, 65°C Sulfurisphaera tokodaii
1.42
-
 Acrylate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Sulfurisphaera tokodaii
Mg2+
-
 Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 2 * 74000 Sulfurisphaera tokodaii
78000
-
 4 * 78000 Metallosphaera sedula
140000
-
 recombinant enzyme, gel filtration Sulfurisphaera tokodaii
340000
-
 gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-hydroxypropanoate + CoA Sulfurisphaera tokodaii
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
ATP + 3-hydroxypropanoate + CoA Metallosphaera sedula
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
ATP + 3-hydroxypropanoate + CoA Metallosphaera sedula DSM 5348
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YGR1
-
-
Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 95fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, anion exchange and adsorption chromatography, followed by gel filtration Metallosphaera sedula
recombinant His-tagged enzyme from Escherichia coli Sulfurisphaera tokodaii

Source Tissue

Source Tissue Comment Organism Textmining
additional information autotrophically grown cells Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
 heterotrophic grown cells Metallosphaera sedula
0.091
-
 autotrophic grown cells Metallosphaera sedula
6.7
-
 purified recombinant enzyme Sulfurisphaera tokodaii
18
-
 purified native enzyme Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-hydroxypropanoate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
ATP + 3-hydroxypropanoate + CoA
-
 Metallosphaera sedula AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
ATP + 3-hydroxypropanoate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
ATP + acetate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acrylate + CoA
-
 Metallosphaera sedula AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + acrylate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + acrylate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + acrylyl-CoA
-
 ?
ATP + butyrate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + butyryl-CoA
-
 ?
ATP + propanoate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA
-
 Metallosphaera sedula AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + propanoyl-CoA
-
 ?
additional information the recombinant enzyme not only activates 3-hydroxypropionate to its CoA ester but also activates propionate, acrylate, acetate, and butyrate with reduced substrates affinities compared to propionate and 3-hydroxypropionate Sulfurisphaera tokodaii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 74000 Sulfurisphaera tokodaii
tetramer 4 * 78000 Metallosphaera sedula

Synonyms

Synonyms Comment Organism
3-hydroxypropionyl-CoA synthetase
-
 Sulfurisphaera tokodaii
3-hydroxypropionyl-CoA synthetase
-
 Metallosphaera sedula
3-hydroxypropionyl-coenzyme A synthetase
-
 Sulfurisphaera tokodaii
3-hydroxypropionyl-coenzyme A synthetase
-
 Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Sulfurisphaera tokodaii
65
-
 assay at Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 Acrylate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
5.6
-
 propanoate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
8
-
 3-hydroxypropanoate pH 8.4, 65°C Sulfurisphaera tokodaii
23
-
 3-hydroxypropanoate pH 8.4, 65°C Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Sulfurisphaera tokodaii
8.4
-
 assay at Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sulfurisphaera tokodaii
ATP
-
 Metallosphaera sedula