BRENDA - Enzyme Database show
show all sequences of 6.2.1.17

Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation

Alber, B.E.; Fuchs, G.; J. Biol. Chem. 277, 12137-12143 (2002)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
CoA
pH 7.8, 55°C
Chloroflexus aurantiacus
0.01
-
NADPH
pH 7.8, 55°C
Chloroflexus aurantiacus
0.015
-
3-hydroxypropionate
pH 7.8, 55°C
Chloroflexus aurantiacus
0.05
-
ATP
pH 7.8, 55°C
Chloroflexus aurantiacus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cs+
can partially substitute for K+
Chloroflexus aurantiacus
K+
required, half aximal activity at 4 mM
Chloroflexus aurantiacus
Mg2+
required
Chloroflexus aurantiacus
NH4+
can partially substitute for K+
Chloroflexus aurantiacus
Rb+
can partially substitute for K+
Chloroflexus aurantiacus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
185000
-
SDS-PAGE
Chloroflexus aurantiacus
500000
800000
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 3-hydroxypropionate + CoA + NADPH
Chloroflexus aurantiacus
involved in CO2 fixation via 3-hydroxypropionate cycle
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chloroflexus aurantiacus
Q8VRG6
-
-
Chloroflexus aurantiacus OK-70-fl / DSM 636
Q8VRG6
-
-
Purification (Commentary)
Commentary
Organism
-
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5
-
purified enzyme, pH 7.8, 55°C
Chloroflexus aurantiacus
Storage Stability
Storage Stability
Organism
-20°C, 20% glycerol, 1 mM dithioerythreitol, stable for several weeks
Chloroflexus aurantiacus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-hydroxypropionate + CoA + NADPH
involved in CO2 fixation via 3-hydroxypropionate cycle
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
ATP + 3-hydroxypropionate + CoA + NADPH
three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
ATP + acrylate + CoA + NADPH
same activity as with 3-hydroxypropionate
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
GTP + 3-hydroxypropionate + CoA + NADPH
24% of activity
656038
Chloroflexus aurantiacus
GMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
additional information
no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate
656038
Chloroflexus aurantiacus
?
-
-
-
-
UTP + 3-hydroxypropionate + CoA + NADPH
20% of activity
656038
Chloroflexus aurantiacus
UMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
homotrimer or homotetramer
Chloroflexus aurantiacus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
stable for at least 15 min in presence of 20% glycerol and 1 mM dithioerythreitol
Chloroflexus aurantiacus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12
-
3-hydroxypropionate
pH 7.8, 55°C
Chloroflexus aurantiacus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
CoA
pH 7.8, 55°C
Chloroflexus aurantiacus
0.01
-
NADPH
pH 7.8, 55°C
Chloroflexus aurantiacus
0.015
-
3-hydroxypropionate
pH 7.8, 55°C
Chloroflexus aurantiacus
0.05
-
ATP
pH 7.8, 55°C
Chloroflexus aurantiacus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cs+
can partially substitute for K+
Chloroflexus aurantiacus
K+
required, half aximal activity at 4 mM
Chloroflexus aurantiacus
Mg2+
required
Chloroflexus aurantiacus
NH4+
can partially substitute for K+
Chloroflexus aurantiacus
Rb+
can partially substitute for K+
Chloroflexus aurantiacus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
185000
-
SDS-PAGE
Chloroflexus aurantiacus
500000
800000
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 3-hydroxypropionate + CoA + NADPH
Chloroflexus aurantiacus
involved in CO2 fixation via 3-hydroxypropionate cycle
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5
-
purified enzyme, pH 7.8, 55°C
Chloroflexus aurantiacus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20% glycerol, 1 mM dithioerythreitol, stable for several weeks
Chloroflexus aurantiacus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-hydroxypropionate + CoA + NADPH
involved in CO2 fixation via 3-hydroxypropionate cycle
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
ATP + 3-hydroxypropionate + CoA + NADPH
three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
ATP + acrylate + CoA + NADPH
same activity as with 3-hydroxypropionate
656038
Chloroflexus aurantiacus
AMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
GTP + 3-hydroxypropionate + CoA + NADPH
24% of activity
656038
Chloroflexus aurantiacus
GMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
additional information
no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate
656038
Chloroflexus aurantiacus
?
-
-
-
-
UTP + 3-hydroxypropionate + CoA + NADPH
20% of activity
656038
Chloroflexus aurantiacus
UMP + diphosphate + NADP+ + propionyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
homotrimer or homotetramer
Chloroflexus aurantiacus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
stable for at least 15 min in presence of 20% glycerol and 1 mM dithioerythreitol
Chloroflexus aurantiacus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12
-
3-hydroxypropionate
pH 7.8, 55°C
Chloroflexus aurantiacus
Other publictions for EC 6.2.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745242
Yoshimura
Molecular cloning of rat acss ...
Rattus norvegicus
J. Biochem.
161
279-289
2017
-
-
1
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3
2
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1
-
3
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4
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1
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1
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1
1
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2
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1
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4
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4
1
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1
-
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1
-
-
746207
Luo
Production of 3-hydroxypropio ...
Escherichia coli
PLoS ONE
11
e0156286
2016
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1
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1
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1
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726798
Yang
Metabolic engineering of Esche ...
Cupriavidus necator, Cupriavidus necator BV3
Appl. Microbiol. Biotechnol.
98
95-104
2014
-
-
1
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2
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7
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2
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728224
Armando
LC-MS/MS quantification of sho ...
Ralstonia solanacearum
Lett. Appl. Microbiol.
54
140-148
2012
-
-
1
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1
1
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3
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1
1
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-
691426
Wong
Engineering poly(3-hydroxybuty ...
Salmonella enterica
Biotechnol. Bioeng.
99
919-928
2008
-
-
1
-
1
-
-
-
-
1
-
1
-
3
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1
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1
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1
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1
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1
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1
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1
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-
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-
-
-
-
692841
Alber
3-Hydroxypropionyl-coenzyme A ...
Metallosphaera sedula, Sulfurisphaera tokodaii
J. Bacteriol.
190
1383-1389
2008
1
-
1
-
-
-
-
7
-
2
4
3
-
8
-
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2
-
-
1
4
-
8
2
2
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4
2
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2
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1
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1
2
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7
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2
4
3
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2
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1
4
-
8
2
2
-
-
4
2
-
-
-
-
-
-
-
-
-
693599
Han
Selective production of epothi ...
Ralstonia solanacearum
J. Microbiol. Biotechnol.
18
135-137
2008
-
-
1
-
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1
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1
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3
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2
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655578
Rajashekhara
Propionyl-coenzyme A synthetas ...
Ralstonia solanacearum, Salmonella enterica
FEBS Lett.
556
143-147
2003
-
-
2
-
-
-
-
12
-
-
4
2
-
2
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2
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16
2
2
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2
12
2
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4
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2
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12
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4
2
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2
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16
2
2
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2
12
2
-
4
-
-
-
-
-
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655852
Palacios
Propionyl coenzyme A is a comm ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
185
2802-2810
2003
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1
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6
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654636
Horswill
Characterization of the propio ...
Salmonella enterica
Biochemistry
41
2379-2387
2002
-
1
1
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7
-
2
8
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1
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3
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1
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4
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4
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8
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656038
Alber
Propionyl-coenzyme A synthase ...
Chloroflexus aurantiacus, Chloroflexus aurantiacus OK-70-fl / DSM 636
J. Biol. Chem.
277
12137-12143
2002
-
-
-
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4
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2
1
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5
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6
1
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1
1
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1
6
1
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1
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656716
Horswill
-
The prpE gene of Salmonella ty ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Microbiology
145
1381-1388
1999
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42
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654
Krahenbuhl
Inhibition of hepatic propiony ...
Rattus norvegicus
Biochem. Pharmacol.
41
1015-1023
1991
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4
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1
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655
Garrastazu
Propionyl-CoA synthetase in ma ...
Bos taurus
Rev. Esp. Fisiol.
47
63-68
1991
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6
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1
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2
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2
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653
Murrell
-
Cell-free assay methods for en ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous PNKb1
Methods Enzymol.
188
26-32
1990
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2
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4
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656
Ricks
Partial purification of enzyme ...
Bos taurus
J. Dairy Sci.
64
2344-2349
1981
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7
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660
Ricks
Regulation of volatile fatty a ...
Bos taurus
J. Dairy Sci.
64
2324-2335
1981
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2
1
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2
1
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2
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1
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
2
1
-
-
-
-
-
1
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658
Reijnierse
Radioassay of acetyl-CoA synth ...
Rattus norvegicus
Anal. Biochem.
72
614-622
1976
-
1
-
-
-
-
3
2
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
2
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
659
Groot
Acyl-CoA synthetases in guinea ...
Cavia porcellus
Biochim. Biophys. Acta
441
260-267
1976
-
-
-
-
-
1
-
2
2
-
-
-
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657
Groot
The activation of short-chain ...
Cavia porcellus
Biochim. Biophys. Acta
380
12-20
1975
-
-
-
-
-
-
3
3
2
-
-
-
-
2
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
2
-
-
-
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
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-
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-