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Literature summary for 6.2.1.17 extracted from

  • Horswill, A.R.; Escalante-Semerena, J.C.
    Characterization of the propionyl-CoA synthetase (PrpE) enzyme of Salmonella enterica: residue Lys592 is required for propionyl-AMP synthesis (2002), Biochemistry, 41, 2379-2387.
    View publication on PubMed

Application

Application Comment Organism
synthesis used for incorporation of unusual acyl groups into biopolymers and polyketide antibiotics Salmonella enterica

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
G245A 58% of activity Salmonella enterica
G249A 11% of activity Salmonella enterica
K248A 12% of activity Salmonella enterica
K248E 6% of activity Salmonella enterica
K592A complete loss of activity Salmonella enterica
K592E complete loss of activity Salmonella enterica
P247A activity almost not affected Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-propyl phosphate strong inhibition, bi uni uni bi ping pong mechanism Salmonella enterica
propyl-AMP competitive with ATP and propionyl-AMP, mixed type inhibition with propionate, uncompetitive with CoA Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
propionate pH 7.5, 37°C Salmonella enterica
0.023
-
propionyl-AMP pH 7.5, 37°C Salmonella enterica
0.057
-
ATP pH 7.5, 37°C Salmonella enterica
0.215
-
CoA pH 7.5, 37°C Salmonella enterica
0.254
-
diphosphate pH 7.5, 37°C Salmonella enterica
0.279
-
Acrylate pH 7.5, 37°C Salmonella enterica
1.7
-
acetate pH 7.5, 37°C Salmonella enterica
5.6
-
Butyrate pH 7.5, 37°C Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + propanoate + CoA Salmonella enterica required for propionate catabolism AMP + diphosphate + propanoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and mutant enzymes using chitin affinity chromatography Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
native enzyme with glycine as substrate, pH 7.5, 37°C Salmonella enterica
0.65
-
native enzyme with glyoxalate as substrate, pH 7.5, 37°C Salmonella enterica
18
-
native enzyme with mercaptoacetoacetate as substrate, pH 7.5, 37°C Salmonella enterica
22
-
native enzyme with propionate as substrate, pH 7.5, 37°C Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA very poor substrate Salmonella enterica AMP + diphosphate + acetyl-CoA
-
?
ATP + acrylate + CoA poor substrate Salmonella enterica AMP + diphosphate + acryloyl-CoA
-
?
ATP + butyrate + CoA very poor substrate Salmonella enterica AMP + diphosphate + butyryl-CoA
-
?
ATP + glycine + CoA very poor substrate Salmonella enterica ?
-
?
ATP + glyoxalate + CoA poor substrate Salmonella enterica ?
-
?
ATP + mercaptoacetoacetate + CoA very good substrate Salmonella enterica AMP + diphosphate + mercaptoacetoacetyl-CoA
-
?
ATP + propanoate + CoA required for propionate catabolism Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
?
ATP + propanoate + CoA via propionyl-AMP intermediate Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
?
propionyl-AMP + CoA
-
Salmonella enterica AMP + propanoyl-CoA
-
ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
Butyrate pH 7.5, 37°C Salmonella enterica
26
-
Acrylate pH 7.5, 37°C Salmonella enterica
32
-
acetate pH 7.5, 37°C Salmonella enterica
33
-
propionate pH 7.5, 37°C Salmonella enterica
34
-
ATP pH 7.5, 37°C Salmonella enterica
42
-
CoA pH 7.5, 37°C Salmonella enterica
78
-
propionyl-AMP pH 7.5, 37°C Salmonella enterica
79
-
diphosphate pH 7.5, 37°C Salmonella enterica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
propyl-AMP competitive with ATP, pH 7.5, 37°C Salmonella enterica