Application | Comment | Organism |
---|---|---|
synthesis | used for incorporation of unusual acyl groups into biopolymers and polyketide antibiotics | Salmonella enterica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
G245A | 58% of activity | Salmonella enterica |
G249A | 11% of activity | Salmonella enterica |
K248A | 12% of activity | Salmonella enterica |
K248E | 6% of activity | Salmonella enterica |
K592A | complete loss of activity | Salmonella enterica |
K592E | complete loss of activity | Salmonella enterica |
P247A | activity almost not affected | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-propyl phosphate | strong inhibition, bi uni uni bi ping pong mechanism | Salmonella enterica | |
propyl-AMP | competitive with ATP and propionyl-AMP, mixed type inhibition with propionate, uncompetitive with CoA | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
propionate | pH 7.5, 37°C | Salmonella enterica | |
0.023 | - |
propionyl-AMP | pH 7.5, 37°C | Salmonella enterica | |
0.057 | - |
ATP | pH 7.5, 37°C | Salmonella enterica | |
0.215 | - |
CoA | pH 7.5, 37°C | Salmonella enterica | |
0.254 | - |
diphosphate | pH 7.5, 37°C | Salmonella enterica | |
0.279 | - |
Acrylate | pH 7.5, 37°C | Salmonella enterica | |
1.7 | - |
acetate | pH 7.5, 37°C | Salmonella enterica | |
5.6 | - |
Butyrate | pH 7.5, 37°C | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + propanoate + CoA | Salmonella enterica | required for propionate catabolism | AMP + diphosphate + propanoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and mutant enzymes using chitin affinity chromatography | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
native enzyme with glycine as substrate, pH 7.5, 37°C | Salmonella enterica |
0.65 | - |
native enzyme with glyoxalate as substrate, pH 7.5, 37°C | Salmonella enterica |
18 | - |
native enzyme with mercaptoacetoacetate as substrate, pH 7.5, 37°C | Salmonella enterica |
22 | - |
native enzyme with propionate as substrate, pH 7.5, 37°C | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | very poor substrate | Salmonella enterica | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acrylate + CoA | poor substrate | Salmonella enterica | AMP + diphosphate + acryloyl-CoA | - |
? | |
ATP + butyrate + CoA | very poor substrate | Salmonella enterica | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + glycine + CoA | very poor substrate | Salmonella enterica | ? | - |
? | |
ATP + glyoxalate + CoA | poor substrate | Salmonella enterica | ? | - |
? | |
ATP + mercaptoacetoacetate + CoA | very good substrate | Salmonella enterica | AMP + diphosphate + mercaptoacetoacetyl-CoA | - |
? | |
ATP + propanoate + CoA | required for propionate catabolism | Salmonella enterica | AMP + diphosphate + propanoyl-CoA | - |
? | |
ATP + propanoate + CoA | via propionyl-AMP intermediate | Salmonella enterica | AMP + diphosphate + propanoyl-CoA | - |
? | |
propionyl-AMP + CoA | - |
Salmonella enterica | AMP + propanoyl-CoA | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
Butyrate | pH 7.5, 37°C | Salmonella enterica | |
26 | - |
Acrylate | pH 7.5, 37°C | Salmonella enterica | |
32 | - |
acetate | pH 7.5, 37°C | Salmonella enterica | |
33 | - |
propionate | pH 7.5, 37°C | Salmonella enterica | |
34 | - |
ATP | pH 7.5, 37°C | Salmonella enterica | |
42 | - |
CoA | pH 7.5, 37°C | Salmonella enterica | |
78 | - |
propionyl-AMP | pH 7.5, 37°C | Salmonella enterica | |
79 | - |
diphosphate | pH 7.5, 37°C | Salmonella enterica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
propyl-AMP | competitive with ATP, pH 7.5, 37°C | Salmonella enterica |