General Stability | Organism |
---|---|
in presence of 10% glycerol the enzyme can be frozen and thawed ten times without loss of activity | Zoogloea ramigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
ATP | - |
Zoogloea ramigera | |
0.076 | - |
acetoacetate | - |
Zoogloea ramigera | |
0.091 | - |
CoA | - |
Zoogloea ramigera | |
1.4 | - |
L(+)-3-hydroxybutyrate | - |
Zoogloea ramigera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace Mg2+ in activation, 26% of the activation relative to Mg2+ | Zoogloea ramigera | |
Cs+ | can replace K+ in activation | Zoogloea ramigera | |
K+ | monovalent cation required | Zoogloea ramigera | |
Mg2+ | - |
Zoogloea ramigera | |
Mn2+ | can replace Mg2+ in activation | Zoogloea ramigera | |
Mn2+ | 96% of the activation relative to Mg2+ | Zoogloea ramigera | |
additional information | absolute requirement for a monovalent cation and a divalent cation | Zoogloea ramigera | |
NH4+ | can replace K+ in activation | Zoogloea ramigera | |
Ni2+ | can replace Mg2+ in activation | Zoogloea ramigera | |
Ni2+ | 21% of the activation relative to Mg2+ | Zoogloea ramigera | |
Rb+ | can replace K+ in activation | Zoogloea ramigera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Zoogloea ramigera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera | - |
- |
- |
Zoogloea ramigera 1-16-M / ATCC 19623 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zoogloea ramigera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52.2 | - |
- |
Zoogloea ramigera |
Storage Stability | Organism |
---|---|
-20°C, 0.07 mg/ml enzyme, 10 mM Tris/HCl buffer, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 1 month | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetoacetate + CoA | r | Zoogloea ramigera | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + acetoacetate + CoA | no activity with GTP, UTP, CTP or ADP | Zoogloea ramigera | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + acetoacetate + CoA | r | Zoogloea ramigera 1-16-M / ATCC 19623 | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + acetoacetate + CoA | no activity with GTP, UTP, CTP or ADP | Zoogloea ramigera 1-16-M / ATCC 19623 | AMP + diphosphate + acetoacetyl-CoA | - |
? | |
ATP + L-3-hydroxybutanoate + CoA | about 15% of the activity relative to ATP | Zoogloea ramigera | AMP + diphosphate + L-3-hydroxybutyryl-CoA | - |
? | |
ATP + L-3-hydroxybutanoate + CoA | about 15% of the activity relative to ATP | Zoogloea ramigera 1-16-M / ATCC 19623 | AMP + diphosphate + L-3-hydroxybutyryl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Zoogloea ramigera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
1 d, 30% loss of activity, in absence of glycerol | Zoogloea ramigera |
30 | - |
1 d, 70% loss of activity, in absence of glycerol | Zoogloea ramigera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
formation of acetoacetate | Zoogloea ramigera |
8.4 | - |
activation of acetoacetate | Zoogloea ramigera |