Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Lysinibacillus sphaericus | |
1,10-phenanthroline | - |
Priestia megaterium | |
2,2'-dipyridyl | - |
Priestia megaterium | |
Al3+ | - |
Priestia megaterium | |
EDTA | - |
Lysinibacillus sphaericus | |
EDTA | - |
Priestia megaterium | |
Fe2+ | - |
Priestia megaterium | |
Fe3+ | - |
Priestia megaterium | |
iodoacetic acid | - |
Priestia megaterium | |
Mg2+ | inhibition above 10 mM, requirement at lower concentrations | Lysinibacillus sphaericus | |
Mg2+ | inhibition above 10 mM, requirement at lower concentrations | Priestia megaterium | |
p-chloromercuribenzoate | - |
Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
heptanedioate | - |
Priestia megaterium | |
0.55 | - |
CoA | - |
Priestia megaterium | |
1.5 | - |
ATP | - |
Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Priestia megaterium | |
Mg2+ | required | Lysinibacillus sphaericus | |
Mg2+ | required | Kocuria rosea | |
Mg2+ | inhibition above 10 mM | Priestia megaterium | |
Mg2+ | inhibition above 10 mM | Lysinibacillus sphaericus | |
Mg2+ | Km: 1.5 mM | Priestia megaterium | |
Mn2+ | - |
Lysinibacillus sphaericus | |
Mn2+ | activates with 50% higher efficiency than Mg2+ | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + heptanedioate + CoA | Bacillus subtilis | - |
? | - |
? | |
ATP + heptanedioate + CoA | Priestia megaterium | enzyme is responsible for the first step in biotin biosynthesis by microorganisms | ? | - |
? | |
ATP + heptanedioate + CoA | Priestia megaterium NIHB 12 | enzyme is responsible for the first step in biotin biosynthesis by microorganisms | ? | - |
? | |
ATP + heptanedioate + CoA | Bacillus subtilis IAM 1193 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
weak activity | - |
Bacillus subtilis IAM 1193 | - |
weak activity | - |
Devosia riboflavina | - |
- |
- |
Devosia riboflavina IFO 3140 | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Enterobacter cloacae IAM 1221 | - |
- |
- |
Escherichia coli | - |
strain AKU 0007 | - |
Klebsiella aerogenes | - |
strain AKU 0022, strain IFO 3317, strain IFO 12010 | - |
Kocuria rosea | - |
- |
- |
Kocuria rosea IAM 1257 | - |
- |
- |
Kocuria rosea IFO 3764 | - |
- |
- |
Lysinibacillus sphaericus | - |
- |
- |
Lysinibacillus sphaericus | - |
weak | - |
Lysinibacillus sphaericus IFO 3525 | - |
- |
- |
Priestia megaterium | - |
- |
- |
Priestia megaterium NIHB 12 | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens AKU 0821 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + heptanedioate + CoA | 85% of the activity relative to ATP | Priestia megaterium | AMP + phosphate + heptanedioyl-CoA | - |
? | |
ADP + heptanedioate + CoA | 85% of the activity relative to ATP | Priestia megaterium NIHB 12 | AMP + phosphate + heptanedioyl-CoA | - |
? | |
ATP + heptanedioate + CoA | - |
Bacillus subtilis | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Escherichia coli | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Enterobacter cloacae | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Priestia megaterium | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Lysinibacillus sphaericus | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Kocuria rosea | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Pseudomonas fluorescens | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Klebsiella aerogenes | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Devosia riboflavina | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Enterobacter cloacae IAM 1221 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Pseudomonas fluorescens AKU 0821 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Priestia megaterium NIHB 12 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Kocuria rosea IFO 3764 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Lysinibacillus sphaericus IFO 3525 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Kocuria rosea IAM 1257 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Bacillus subtilis IAM 1193 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Devosia riboflavina IFO 3140 | AMP + diphosphate + heptanedioyl-CoA | heptanedioyl-CoA i.e. pimelyl-CoA | ? | |
ATP + heptanedioate + CoA | - |
Bacillus subtilis | ? | - |
? | |
ATP + heptanedioate + CoA | enzyme is responsible for the first step in biotin biosynthesis by microorganisms | Priestia megaterium | ? | - |
? | |
ATP + heptanedioate + CoA | enzyme is responsible for the first step in biotin biosynthesis by microorganisms | Priestia megaterium NIHB 12 | ? | - |
? | |
ATP + heptanedioate + CoA | - |
Bacillus subtilis IAM 1193 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 52 | 15°C: about 50% of maximal activity, 52°C: about 15% of maximal activity | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris-HCl buffer | Priestia megaterium |