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Literature summary for 6.2.1.13 extracted from

  • Awano, T.; Wilming, A.; Tomita, H.; Yokooji, Y.; Fukui, T.; Imanaka, T.; Atomia, H.
    Characterization of two members among the five ADP-forming acyl coenzyme a (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis (2014), J. Bacteriol., 196, 140-147 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
CoA at pH 6.5 and 55°C Thermococcus kodakarensis
0.0556
-
ATP at pH 6.5 and 55°C Thermococcus kodakarensis
0.132
-
Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
0.322
-
thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
0.329
-
phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
1.5
-
acetate at pH 6.5 and 55°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Thermococcus kodakarensis
-
ADP + phosphate + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIA8 and Q5JIA9
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource Q column chromatography and Superdex 200 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methylbutyrate + CoA
-
Thermococcus kodakarensis ADP + phosphate + 2-methylbutyryl-CoA
-
?
ATP + 3-methylthiopropionate + CoA
-
Thermococcus kodakarensis ADP + phosphate + 3-methylthiopropionyl-CoA
-
?
ATP + acetate + CoA
-
Thermococcus kodakarensis ADP + phosphate + acetyl-CoA
-
?
ATP + isobutyrate + CoA
-
Thermococcus kodakarensis ADP + phosphate + isobutyryl-CoA
-
?
ATP + isovalerate + CoA
-
Thermococcus kodakarensis ADP + phosphate + isovaleryl-CoA
-
?
ATP + phenylacetate + CoA
-
Thermococcus kodakarensis ADP + phosphate + phenylacetyl-CoA
-
?
ATP + thioglycolate + CoA
-
Thermococcus kodakarensis ADP + phosphate + thioglycolyl-CoA
-
?
additional information almost no activity with formate, 5-aminovalerate, 4-guanidinobutyrate, 2-(imidazol-4-yl)acetate, malonate, and succinate Thermococcus kodakarensis ?
-
?

Synonyms

Synonyms Comment Organism
ACS III isoform Thermococcus kodakarensis
ADP-forming acyl coenzyme A synthetase
-
Thermococcus kodakarensis
ADP-forming acyl-CoA synthetase
-
Thermococcus kodakarensis
TK0944/TK0943 protein
-
Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.1
-
thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
40.6
-
phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
49.3
-
acetate at pH 6.5 and 55°C Thermococcus kodakarensis
50.9
-
Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
53.1
-
CoA at pH 6.5 and 55°C Thermococcus kodakarensis
53.7
-
ATP at pH 6.5 and 55°C Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
32.8
-
acetate at pH 6.5 and 55°C Thermococcus kodakarensis
81
-
thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
123
-
phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
385
-
Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
965
-
ATP at pH 6.5 and 55°C Thermococcus kodakarensis
9490
-
CoA at pH 6.5 and 55°C Thermococcus kodakarensis