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Literature summary for 6.2.1.13 extracted from
- Acetyl coenzyme A synthetase (ADP forming) from the hyperthermophilic Archaeon pyrococcus furiosus: identification, cloning, separate expression of the encoding genes, acdAI and acdBI, in Escherichia coli, and in vitro reconstitution of the active heterotetrameric enzyme from its recombinant subunits (1999), J. Bacteriol., 181, 5885-5888.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Giardia intestinalis |
| overexpression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
acetyl-CoA | - |
Pyrococcus furiosus |  |
| 0.021 | - |
CoA | - |
Pyrococcus furiosus | |
| 0.06 | - |
ADP | - |
Pyrococcus furiosus | |
| 0.06 | - |
acetyl-CoA | pH 7.5, at 30ºC | Giardia intestinalis | |
| 0.09 | - |
ATP | - |
Pyrococcus furiosus | |
| 0.1 | - |
phosphate | - |
Pyrococcus furiosus | |
| 0.2 | - |
ADP | pH 7.5, at 30ºC | Giardia intestinalis | |
| 0.8 | - |
acetate | - |
Pyrococcus furiosus | |
| 1.35 | - |
phosphate | pH 7.5, at 30ºC | Giardia intestinalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Giardia intestinalis | - |
- |
| soluble | - |
Pyrococcus furiosus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | requirement for a divalent cation | Giardia intestinalis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
| 47000 | - |
alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
| 145000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | Giardia intestinalis | enzyme catalyzes acetate formation and ATP generation during fermentation of pyruvate to acetate | ATP + acetate + CoA | - |
? | |
| ADP + phosphate + acetyl-CoA | Pyrococcus furiosus | major energy-conserving reaction during pyruvate and sugar conversion to acetate, catalyzing acetate formation and ATP synthesis | ATP + acetate + CoA | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Giardia intestinalis | Q9Y1N2 | - |
- |
| Pyrococcus furiosus | Q9Y8L0 | subunit beta; isoenzyme ACS I | - |
| Pyrococcus furiosus | Q9Y8L1 | subunit alpha; isoenzyme ACS I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 10fold, 15 min at 80ºC and subsequent anion-exchange chromatography | Pyrococcus furiosus |
| ion-exchange chromatography | Giardia intestinalis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| equal amounts of Escherichia coli extracts containing alpha and beta subunits separately expressed mixed and incubated on ice | Pyrococcus furiosus |
| equal amounts of Escherichia coli extracts containing alpha and beta subunits separately expressed, mixed and incubated on ice | Pyrococcus furiosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Pyrococcus furiosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA | - |
Pyrococcus furiosus | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA | no activity with butanoyl-CoA, propanoyl-CoA and pentanoyl-CoA | Giardia intestinalis | ATP + acetate + CoA | - |
? | |
| ADP + phosphate + acetyl-CoA | enzyme catalyzes acetate formation and ATP generation during fermentation of pyruvate to acetate | Giardia intestinalis | ATP + acetate + CoA | - |
? | |
| ADP + phosphate + acetyl-CoA | major energy-conserving reaction during pyruvate and sugar conversion to acetate, catalyzing acetate formation and ATP synthesis | Pyrococcus furiosus | ATP + acetate + CoA | - |
r | |
| ADP + phosphate + butanoyl-CoA | 23% of the activity relative to acetyl-CoA | Giardia intestinalis | ATP + butanoate + CoA | - |
? | |
| ADP + phosphate + propanoyl-CoA | 45% of the activity relative to acetyl-CoA | Giardia intestinalis | ATP + propanoate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
| monomer | - |
Giardia intestinalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-coenzyme A synthetase (ADP-forming) | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 87 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
80-110ºC, no lose activity upon incubation for 3h at 90ºC, lose about 60% after 2h at 100ºC | Pyrococcus furiosus |
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