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Literature summary for 6.2.1.12 extracted from

  • Chowdhury, M.; Choi, B.; Cho, B.; Kim, J.; Park, S.; Natarajan, S.; Lim, H.; Bae, H.
    Regulation of 4CL, encoding 4-coumarate: coenzyme a ligase, expression in kenaf under diverse stress conditions (2013), Plant OMICS, 6, 254-262.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, quantitative real-time PCR expression analysis Hibiscus cannabinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59560
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x * 59560, sequence calculation Hibiscus cannabinus

Organism

Organism UniProt Comment Textmining
Hibiscus cannabinus T1RK64
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-

Source Tissue

Source Tissue Comment Organism Textmining
flower mature, low expression level Hibiscus cannabinus
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leaf low expression level Hibiscus cannabinus
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additional information differential expression in all tissues during the developmental stages, transcript expression patterns, quantitative real-time PCR analysis, overview Hibiscus cannabinus
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root high expression level Hibiscus cannabinus
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stem high expression level Hibiscus cannabinus
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Subunits

Subunits Comment Organism
? x * 59560, sequence calculation Hibiscus cannabinus

Synonyms

Synonyms Comment Organism
4-coumarate: coenzyme A ligase
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Hibiscus cannabinus
4CL
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Hibiscus cannabinus
Hc4CL
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Hibiscus cannabinus

Cofactor

Cofactor Comment Organism Structure
ATP
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Hibiscus cannabinus

pI Value

Organism Comment pI Value Maximum pI Value
Hibiscus cannabinus sequence calculation
-
6.58

Expression

Organism Comment Expression
Hibiscus cannabinus the enzyme expression is reduced by abscisic acid treatment. The Hc4CL gene is downregulated at early time points and then upregulated 24 h after H2O2 treatment, after which the transcript level returns to a negative level relative to the control. The enzyme also shows biphasic expression in response to wounding. Specifically, the transcript is highly expressed at 6 h and then decreases sharply at 12 h with a second induction at 24 h down
Hibiscus cannabinus Hc4CL is upregulated by NaCl treatment, reaching its maximum level at 24 h after treatment. The enzyme is induced by cold and drought treatment. The Hc4CL gene is downregulated at early time points and then upregulated 24 h after H2O2 treatment, after which the transcript level returns to a negative level relative to the control. The enzyme also shows biphasic expression in response to wounding. Specifically, the transcript is highly expressed at 6 h and then decreases sharply at 12 h with a second induction at 24 h up

General Information

General Information Comment Organism
additional information the enzyme has two conserved putative AMP-binding motifs, the SSGTTGLPKGV and GEICIRG domains Hibiscus cannabinus
physiological function the enzyme is a key regulatory enzyme of the phenylpropanoid pathway that regulates the activation of cinnamic acid, leading to the synthesis of flavonoids and lignin Hibiscus cannabinus